SARDH rabbit pAb
- Catalog No.:YT7522
- Applications:WB;ELISA;IHC
- Reactivity:Human;Mouse;Rat
- Target:
- SARDH
- Fields:
- >>Glycine, serine and threonine metabolism;>>Metabolic pathways
- Gene Name:
- SARDH DMGDHL1
- Protein Name:
- SARDH
- Human Gene Id:
- 1757
- Human Swiss Prot No:
- Q9UL12
- Mouse Gene Id:
- 192166
- Mouse Swiss Prot No:
- Q99LB7
- Rat Gene Id:
- 114123
- Rat Swiss Prot No:
- Q64380
- Immunogen:
- Synthesized peptide derived from human SARDH AA range: 279-329
- Specificity:
- This antibody detects endogenous levels of SARDH at Human/Mouse/Rat
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Molecular Weight(Da):
- 101kD
- Background:
- This gene encodes an enzyme localized to the mitochondrial matrix which catalyzes the oxidative demethylation of sarcosine. This enzyme is distinct from another mitochondrial matrix enzyme, dimethylglycine dehydrogenase, which catalyzes a reaction resulting in the formation of sarcosine. Mutations in this gene are associated with sarcosinemia. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2008],
- Function:
- catalytic activity:Sarcosine + acceptor + H(2)O = glycine + formaldehyde + reduced acceptor.,cofactor:Binds 1 FAD covalently per monomer.,disease:Defects in SARDH are a ause of sarcosinemia [MIM:268900]. Sarcosinemia is a metabolic disorder characterized by an increased concentration of sarcosine in plasma and urine due to sarcosine dehydrogenase deficiency. Prevalence has been estimated at 1:28'000 to 1:350'000 in newborn screening programs. Sarcosinemia is most probably a benign condition without significant clinical problems. It is transmitted in an autosomal recessive manner.,pathway:Amine and polyamine degradation; sarcosine degradation; formaldehyde and glycine from sarcosine: step 1/1.,similarity:Belongs to the gcvT family.,
- Subcellular Location:
- Mitochondrion matrix .
- Expression:
- Expressed in pancreas, liver and kidney.
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- Antibody-FAQs
- Products Images
- Western blot analysis of lysates from 3T3 cells, primary antibody was diluted at 1:1000, 4°over night
- Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).