MTDC rabbit pAb
- Catalog No.:YT6697
- Applications:WB;IHC
- Reactivity:Human;Mouse
- Target:
- MTDC
- Fields:
- >>One carbon pool by folate;>>Metabolic pathways;>>Biosynthesis of cofactors
- Gene Name:
- MTHFD2 NMDMC
- Protein Name:
- MTDC
- Human Gene Id:
- 10797
- Human Swiss Prot No:
- P13995
- Mouse Gene Id:
- 17768
- Mouse Swiss Prot No:
- P18155
- Immunogen:
- Synthesized peptide derived from human MTDC AA range: 121-171
- Specificity:
- This antibody detects endogenous levels of MTDC at Human/Mouse
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500-2000;IHC 1:50-300
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Molecular Weight(Da):
- 39kD
- Background:
- This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq, Mar 2009],
- Function:
- catalytic activity:5,10-methenyltetrahydrofolate + H(2)O = 10-formyltetrahydrofolate.,catalytic activity:5,10-methylenetetrahydrofolate + NAD(+) = 5,10-methenyltetrahydrofolate + NADH.,cofactor:Magnesium.,developmental stage:Expressed only in developing normal tissues.,miscellaneous:This NAD-dependent bifunctional enzyme has very different kinetic properties than the larger NADP-dependent trifunctional enzyme and is unique in that it requires formation of an enzyme-magnesium complex to allow binding of NAD.,similarity:Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.,subunit:Homodimer.,
- Subcellular Location:
- Mitochondrion.
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- Antibody-FAQs
- Products Images
- Western blot analysis of lysates from 293T cells, primary antibody was diluted at 1:1000, 4°over night
- Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).