ATG3 rabbit pAb
- Catalog No.:YT6685
- Applications:WB
- Reactivity:Human;Mouse;Rat
- Target:
- ATG3
- Fields:
- >>Autophagy - other;>>Autophagy - animal;>>Kaposi sarcoma-associated herpesvirus infection
- Gene Name:
- ATG3 APG3 APG3L
- Protein Name:
- ATG3
- Human Gene Id:
- 64422
- Human Swiss Prot No:
- Q9NT62
- Mouse Gene Id:
- 67841
- Mouse Swiss Prot No:
- Q9CPX6
- Rat Gene Id:
- 171415
- Rat Swiss Prot No:
- Q6AZ50
- Immunogen:
- Synthesized peptide derived from human ATG3 AA range: 28-78
- Specificity:
- This antibody detects endogenous levels of ATG3 at Human/Mouse/Rat
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500-2000
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Molecular Weight(Da):
- 35kD
- Background:
- This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013],
- Function:
- function:GABARAPL1 (GABARAPL2 or GABARAP or MAP1LC3)-modifier protein conjugating enzyme involved in its E2-like covalent binding to PE. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (E2-like enzyme). Preferred substrate is MAP1LC3A. Formation of the GABARAPL1-PE conjugate is essential for autophagy.,similarity:Belongs to the ATG3 family.,subunit:Interacts with ATG7 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5.,tissue specificity:Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta.,
- Subcellular Location:
- Cytoplasm .
- Expression:
- Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta.
- June 19-2018
- WESTERN IMMUNOBLOTTING PROTOCOL
- June 19-2018
- IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL
- June 19-2018
- IMMUNOFLUORESCENCE PROTOCOL
- September 08-2020
- FLOW-CYTOMEYRT-PROTOCOL
- May 20-2022
- Cell-Based ELISA│解您多样本WB检测之困扰
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN
- July 13-2018
- Antibody-FAQs
- Products Images
- Western blot analysis of lysates from HEK293 cells, primary antibody was diluted at 1:1000, 4°over night