ATG4A Polyclonal Antibody
- Catalog No.:YT6148
- Applications:WB;ELISA
- Reactivity:Human;Mouse;Rat
- Target:
- ATG4A
- Fields:
- >>Autophagy - other;>>Autophagy - animal
- Gene Name:
- ATG4A APG4A AUTL2
- Protein Name:
- Cysteine protease ATG4A (EC 3.4.22.-) (AUT-like 2 cysteine endopeptidase) (Autophagin-2) (Autophagy-related cysteine endopeptidase 2) (Autophagy-related protein 4 homolog A) (hAPG4A)
- Human Gene Id:
- 115201
- Human Swiss Prot No:
- Q8WYN0
- Mouse Gene Id:
- 666468
- Mouse Swiss Prot No:
- Q8C9S8
- Immunogen:
- Synthesized peptide derived from human ATG4A Polyclonal
- Specificity:
- This antibody detects endogenous levels of ATG4A.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500-2000, ELISA 1:10000-20000
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- Cysteine protease ATG4A (EC 3.4.22.-) (AUT-like 2 cysteine endopeptidase) (Autophagin-2) (Autophagy-related cysteine endopeptidase 2) (Autophagy-related protein 4 homolog A) (hAPG4A)
- Observed Band(KD):
- 40kD
- Background:
- Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. [provided by RefSeq, Mar 2016],
- Function:
- enzyme regulation:Inhibited by N-ethylmaleimide.,function:Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP.,similarity:Belongs to the peptidase C54 family.,tissue specificity:Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver.,
- Subcellular Location:
- Cytoplasm .
- Expression:
- Epithelium,Kidney,Ovary,Prostate,Testis,
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- Antibody-FAQs
- Products Images
- Western blot analysis of mouse-liver lysate, antibody was diluted at 1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000