• Target：
• MCAD

• Fields：
• >>Fatty acid degradation;>>Valine, leucine and isoleucine degradation;>>Metabolic pathways;>>Fatty acid metabolism;>>PPAR signaling pathway;>>Alcoholic liver disease

• Gene Name：
• ACADM

• Protein Name：
• Medium-chain specific acyl-CoA dehydrogenase mitochondrial

• Human Gene Id：
• 34

• Human Swiss Prot No：
• P11310

• Mouse Gene Id：
• 11364

• Mouse Swiss Prot No：
• P45952

• Rat Gene Id：
• 24158

• Rat Swiss Prot No：
• P08503

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human MCAD. AA range:134-183

• Specificity：
• MCAD Polyclonal Antibody detects endogenous levels of MCAD protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500-2000;IHC 1:50-300

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• ACADM;Medium-chain specific acyl-CoA dehydrogenase, mitochondrial;MCAD

• Observed Band(KD)：
• 46kD

• Background：
• This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

• Function：
• catalytic activity:Acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor.,cofactor:FAD.,disease:Defects in ACADM are the cause of medium-chain acyl-CoA dehydrogenase deficiency (MCAD deficiency) [MIM:201450]. It is an autosomal recessive disease which causes fasting hypoglycemia, hepatic dysfunction, and encephalopathy, often resulting in death in infancy. The disease frequency is one in 13000.,function:This enzyme is specific for acyl chain lengths of 4 to 16.,miscellaneous:A number of straight-chain acyl-CoA dehydrogenases of different substrate specificities are present in mammalian tissues.,miscellaneous:Utilizes the electron transfer flavoprotein (ETF) as electron acceptor that transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).,pathway:Lipid metabolism; mitochondrial fatty acid beta-oxidation.,similarity:

• Subcellular Location：
• Mitochondrion matrix .

• Expression：
• Brain,Cajal-Retzius cell,Cerebellum,Colon,Liver,

Bifidobacterium animalis subsp. lactis A6 Enhances Fatty Acid β-Oxidation of Adipose Tissue to Ameliorate the Development of Obesity in Mice Nutrients. 2022 Jan;14(3):598. WB Mouse epididymal adipose tissues

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