NEEP21 Polyclonal Antibody

    • Catalog No.:YT3028
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • NEEP21
      • Gene Name:
      • NSG1
      • Protein Name:
      • Neuron-specific protein family member 1
      • Human Swiss Prot No:
      • P42857
      • Mouse Swiss Prot No:
      • Q62092
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human NSG1. AA range:112-161
      • Specificity:
      • NEEP21 Polyclonal Antibody detects endogenous levels of NEEP21 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • NSG1;D4S234;Neuron-specific protein family member 1;Brain neuron cytoplasmic protein 1
      • Observed Band(KD):
      • 26kD
      • Background:
      • similarity:Belongs to the NSG family.,
      • Function:
      • similarity:Belongs to the NSG family.,
      • Subcellular Location:
      • Membrane ; Single-pass type II membrane protein . Golgi apparatus, trans-Golgi network membrane . Endosome membrane . Cell projection, dendrite . Early endosome membrane . Late endosome membrane . Lysosome lumen . Recycling endosome membrane . Cytoplasmic vesicle membrane . Golgi apparatus, Golgi stack membrane . Endosome, multivesicular body membrane . Endoplasmic reticulum membrane . Endocytosed from the cell surface, thus enters into early endosomes, trafficks to late endosomes and degradates in lysosomes (By similarity). Endoplasmic reticulum targeting is essential for apoptosis (PubMed:20599942). Found in both stationary and motile endosomes. A previous study supports a type I membrane protein topology (By similarity). .
      • Expression:
      • Amygdala,Brain,Cerebellum,Testis,
      • Products Images
      • Western Blot analysis of various cells using NEEP21 Polyclonal Antibody diluted at 1:2000
      • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
      • Western blot analysis of lysates from COLO cells, using NSG1 Antibody. The lane on the right is blocked with the synthesized peptide.
      • Western blot analysis of the lysates from K562 cells using NSG1 antibody.