CAD (Phospho Ser1859) rabbit pAb

  • Catalog No.：YP1284
  • Applications：WB
  • Reactivity：Human;Mouse;Rat
    • Target：
    • CAD
    • Fields：
    • >>Pyrimidine metabolism;>>Alanine, aspartate and glutamate metabolism;>>Metabolic pathways;>>Biosynthesis of cofactors
    • Gene Name：
    • CAD
    • Protein Name：
    • CAD (Ser1859)
    • Human Gene Id：
    • 790
    • Human Swiss Prot No：
    • P27708
    • Immunogen：
    • Synthesized phosho peptide around human CAD (Ser1859)
    • Specificity：
    • This antibody detects endogenous levels of Human Mouse Rat CAD (phospho-Ser1859)
    • Formulation：
    • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
    • Source：
    • Polyclonal, Rabbit,IgG
    • Dilution：
    • WB 1:1000-2000
    • Purification：
    • The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
    • Concentration：
    • 1 mg/ml
    • Storage Stability：
    • -15°C to -25°C/1 year(Do not lower than -25°C)
    • Other Name：
    • CAD protein [Includes: Glutamine-dependent carbamoyl-phosphate synthase (EC 6.3.5.5);Aspartate carbamoyltransferase (EC 2.1.3.2);Dihydroorotase (EC 3.5.2.3)]
    • Observed Band(KD)：
    • 250kD
    • Background：
    • The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015],
    • Function：
    • catalytic activity:(S)-dihydroorotate + H(2)O = N-carbamoyl-L-aspartate.,catalytic activity:2 ATP + L-glutamine + HCO(3)(-) + H(2)O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.,catalytic activity:Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate.,cofactor:Binds 1 zinc ion per subunit (for dihydroorotase activity) .,enzyme regulation:Allosterically regulated and controlled by phosphorylation. 5-phosphoribose 1-diphosphate is an activator while UMP is an inhibitor of the CPSase reaction.,function:This protein is a "fusion" protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase).,miscellaneous:GATase (glutamine amidotransferase) and CPSase (carbamoyl phosphate synthase) form together the glutamine-dependent CPSase (GD-CPSase) (EC 6.3.5.5).,online information:Aspartate carbamoyltransferase entry,pathway:Pyrimi
    • Subcellular Location：
    • Cytoplasm . Nucleus . Cytosolic and unphosphorylated in resting cells, translocates to the nucleus in response to EGF stimulation, nuclear import promotes optimal cell growth.
    • Expression：
    • Colon adenocarcinoma,Epithe

    Attributes

    • Code：
    • YP1284
    • Size
    • 50μL / 100μL / 200μL
    • Price$：
    • 190 / 340 / 600
    • Delivery：
    • 2024-07-17
    Order

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