• Target：
• MLKL

• Fields：
• >>Necroptosis;>>TNF signaling pathway;>>Salmonella infection

• Gene Name：
• MLKL

• Protein Name：
• MLKL (phospho S358)

• Human Gene Id：
• 197259

• Human Swiss Prot No：
• Q8NB16

• Immunogen：
• Synthesized peptide derived from human MLKL (phospho S358)

• Specificity：
• This antibody detects endogenous phospho levels of MLKL (phospho S358) at Human, Mouse, Rat

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• IHC 1:100-500;IF ICC 1:100-500

• Purification：
• The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• Mixed lineage kinase domain-like protein

• Observed Band(KD)：
• 55kD

• Background：
• This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015],

• Function：
• domain:The protein kinase domain is predicted to be catalytically inactive.,similarity:Belongs to the protein kinase superfamily.,similarity:Contains 1 protein kinase domain.,

• Subcellular Location：
• Cytoplasm . Cell membrane . Nucleus . Localizes to the cytoplasm and translocates to the plasma membrane on necroptosis induction (PubMed:24316671). Localizes to the nucleus in response to orthomyxoviruses infection (By similarity). .

• Expression：
• Chondrocyte,Leukocyte,Lymph node,

IMB5036 inhibits human pancreatic cancer growth primarily through activating necroptosis. BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY Basic Clin Pharmacol. 2022 Mar;130(3):375-384 IF Human Capan-2 cell，BxPC-3 cell

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