• Target：
• DAPK1

• Fields：
• >>Autophagy - animal;>>Pathways in cancer;>>Bladder cancer

• Gene Name：
• DAPK1 DAPK

• Protein Name：
• DAPK1 (Phospho-Ser308)

• Human Gene Id：
• 1612

• Human Swiss Prot No：
• P53355

• Immunogen：
• Synthesized peptide derived from human DAPK1 (Phospho-Ser308)

• Specificity：
• This antibody detects endogenous phospho levels of DAPK1 (Phospho-Ser308) at Human:S308, Mouse:S308

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• IHC 1:50-200, WB 1:500-2000. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• Death-associated protein kinase 1 (DAP kinase 1;EC 2.7.11.1)

• Observed Band(KD)：
• 160kD

• Background：
• Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013],

• Function：
• catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Negatively regulated by autophosphorylation on Ser-308.,function:Calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis.,induction:Up-regulated following treatment with interferon-gamma.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.,similarity:Contains 1 death domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 10 ANK repeats.,subcellular location:Colocalizes with the actin filament system.,

• Subcellular Location：
• [Isoform 1]: Cytoplasm. Cytoplasm, cytoskeleton. Colocalizes with MAP1B in the microtubules and cortical actin fibers.; [Isoform 2]: Cytoplasm. Cytoplasm, cytoskeleton.

• Expression：
• Isoform 2 is expressed in normal intestinal tissue as well as in colorectal carcinomas.

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