MARK1/2/3/4 (phospho Thr215) Polyclonal Antibody
- Catalog No.:YP1008
- Applications:IHC;IF;ELISA
- Reactivity:Human;Mouse;Rat
- Target:
- MARK1/2/3/4
- Gene Name:
- MARK1/2/3/4
- Protein Name:
- Serine/threonine-protein kinase MARK1/2/3/4
- Human Gene Id:
- 4139/2011/4140/57787
- Human Swiss Prot No:
- Q9P0L2/Q7KZI7/P27448/Q96L34
- Mouse Gene Id:
- 226778/13728/17169/232944
- Rat Gene Id:
- 117016/60328/170577
- Rat Swiss Prot No:
- O08678/O08679/Q8VHF0
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human MARK1/2/3/4 around the phosphorylation site of Thr215. AA range:181-230
- Specificity:
- Phospho-MARK1/2/3/4 (T215) Polyclonal Antibody detects endogenous levels of MARK1/2/3/4 protein only when phosphorylated at T215.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- MARK1;KIAA1477;MARK;Serine/threonine-protein kinase MARK1;MAP/microtubule affinity-regulating kinase 1;PAR1 homolog c;Par-1c;Par1c;MARK2;EMK1;Serine/threonine-protein kinase MARK2;ELKL motif kinase 1;EMK-1;MAP/microtubule affin
- Molecular Weight(Da):
- 89kD
- Background:
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Activated by phosphorylation on Thr-215 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39.,function:May play a role in cytoskeletal stability.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily.,similarity:Contains 1 KA1 (kinase-associated) domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 UBA domain.,subcellular location:Appears to localize to an intracellular network.,tissue specificity:Highly expressed in heart, skeletal muscle, brain, fetal brain and fetal kidney.,
- Function:
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Activated by phosphorylation on Thr-215 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39.,function:May play a role in cytoskeletal stability.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily.,similarity:Contains 1 KA1 (kinase-associated) domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 UBA domain.,subcellular location:Appears to localize to an intracellular network.,tissue specificity:Highly expressed in heart, skeletal muscle, brain, fetal brain and fetal kidney.,
- Subcellular Location:
- Cell membrane ; Peripheral membrane protein . Cytoplasm, cytoskeleton . Cytoplasm . Cell projection, dendrite . Appears to localize to an intracellular network. .
- Expression:
- Highly expressed in heart, skeletal muscle, brain, fetal brain and fetal kidney.
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- Antibody-FAQs
- Products Images
- Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MARK1/2/3/4 (Phospho-Thr215) Antibody
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).