• Target：
• P21

• Gene Name：
• CDKN1A CAP20 CDKN1 CIP1 MDA6 PIC1 SDI1 WAF1

• Protein Name：
• p21

• Human Gene Id：
• 1026

• Human Swiss Prot No：
• P38936

• Mouse Gene Id：
• 12575

• Mouse Swiss Prot No：
• P39689

• Specificity：
• endogenous

• Formulation：
• PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

• Source：
• Monoclonal, rabbit, IgG, Kappa

• Dilution：
• IHC 1:100-1:200,WB 1:1000-1:5000,IF 1:200-1:1000,ELISA 1:5000-1:20000,IP 1:50-1:200,

• Purification：
• Protein A

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• Cyclin-dependent kinase inhibitor 1 ;CDK-interacting protein 1;Melanoma differentiation-associated protein 6;MDA-6;p21;

• Molecular Weight(Da)：
• 18kD

• Observed Band(KD)：
• 18kD

• Function：
• function:May be the important intermediate by which p53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression.,induction:By p53, mezerein (antileukemic compound) and interferon beta.,PTM:Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA.,similarity:Belongs to the CDI family.,tissue specificity:Expressed in all adult human tissues, with 5-fold lower levels observed in the brain.,

• Subcellular Location：
• Nucleus

• Expression：
• Expressed in all adult tissues, with 5-fold lower levels observed in the brain.

Exosomal transfer of miR-181b-5p confers senescence-mediated doxorubicin resistance via modulating BCLAF1 in breast cancer BRITISH JOURNAL OF CANCER Zhao, Shaorong, Pan, Teng, Deng, Jinhai, Cao, Lixia, Vicencio, Jose M., Liu, Jingjing, Zhou, Guanglin, Ng, Tony, Zhang, Jin WB Human, Mouse Cal51 cell-xenograft, CALDOX cell-xenograft Cal51 cell, MCF-7 cell

Chidamide Combined With Doxorubicin Induced p53-Driven Cell Cycle Arrest and Cell Apoptosis Reverse Multidrug Resistance of Breast Cancer. Frontiers in Oncology Front Oncol. 2021 Mar;0:344 WB Human 1:1000 CALDOX cell，MCF-7 cell，A02 cell

Roles of eIF3m in the tumorigenesis of triple negative breast cancer. Cancer Cell International Cancer Cell Int. 2020 Dec;20(1):1-16 WB Human 1:500 MDA-MB-231 cell, MDA-MB-436,MCF-10A cell

Ginsenoside Rg1 improves fertility and reduces ovarian pathological damages in premature ovarian failure model of mice:. EXPERIMENTAL BIOLOGY AND MEDICINE Exp Biol Med. 2017;242(7):683-691 WB Mouse ovaries

Irisin ameliorates nicotine-mediated atherosclerosis via inhibition of the PI3K pathway. Annals of Translational Medicine Ann Transl Med. 2021 May; 9(9): 805 WB Mouse 1:1000 Aortic

Suppression of CDCA3 inhibits prostate cancer progression via NF‑κB/cyclin D1 signaling inactivation and p21 accumulation. ONCOLOGY REPORTS Oncol Rep. 2022 Feb;47(2):1-10 WB Human 1:1000 Prostate cancer tissue,paracancerous tissue.

Sirtuin 7 promotes non‑small cell lung cancer progression by facilitating G1/S phase and epithelial‑mesenchymal transition and activating AKT and ERK1/2 signaling. ONCOLOGY REPORTS Oncol Rep. 2020 Sep;44(3):959-972 WB,IHC Human 1 : 1000,1:200 Lung cancer TMA section A549 cell, H292 cell

ZIC1 acts a tumor suppressor in breast cancer by targeting survivin. INTERNATIONAL JOURNAL OF ONCOLOGY Int J Oncol. 2018 Sep;53(3):937-948 WB Human 1:500 breast tumors MDA-MB-231 cell, SK-BR3 cell

Capsaicin inhibits proliferation and induces apoptosis in osteosarcoma cell lines via the mitogen‑activated protein kinase pathway. ONCOLOGY REPORTS 2017 Nov 01 WB Human MG63 cell,143B cell, HOS cell

Irisin ameliorates nicotine-mediated atherosclerosis via inhibition of the PI3K pathway. Annals of Translational Medicine Ann Transl Med. 2021 May; 9(9): 805 WB Mouse 1:10000 Aortic

Chidamide Combined With Doxorubicin Induced p53-Driven Cell Cycle Arrest and Cell Apoptosis Reverse Multidrug Resistance of Breast Cancer. Frontiers in Oncology Front Oncol. 2021 Mar;0:344

Irisin ameliorates nicotine-mediated atherosclerosis via inhibition of the PI3K pathway. Annals of Translational Medicine Ann Transl Med. 2021 May; 9(9): 805 WB Mouse 1:10000 Aortic

Chidamide Combined With Doxorubicin Induced p53-Driven Cell Cycle Arrest and Cell Apoptosis Reverse Multidrug Resistance of Breast Cancer. Frontiers in Oncology Front Oncol. 2021 Mar;0:344

sST2: A Bridge Between Sirt1/p53/p21 Signal-Induced Senescence and TGF-β1/Smad2/3 Regulation of Cardiac Fibrosis in Mouse Viral Myocarditis INFLAMMATION Lu Hongxiang WB,IF Mouse Heart mouse cardiac fibroblasts (MCFs)

Identification of the Kupffer cell-derived circulating IGFBP-3 as a universal radiation biomarker for heavy ion, proton, and X-ray exposure. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Wenjun Wei WB Mouse 1:3000 mice Kupffer cell(MKC)

Protective effect of cornuside on OGD/R injury in SH-SY5Y cells and its underlying mechanism. BRAIN RESEARCH Jianwei Gong WB Human SH-SY5Y cell

L-Theanine delays D-galactose-induced senescence by regulating the cell cycle and inhibiting apoptosis in rat intestinal cells JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE Wei Xu WB Rat 1:1000 IEC-6 cell

Paeonol Attenuates Atherosclerosis by Inhibiting Vascular Smooth Muscle Cells Senescence via SIRT1/P53/TRF2 Signaling Pathway MOLECULES Min Zhou WB Mouse 1:2000 aorta tissue Vascular smooth muscle cell (VSMC)

Modulation of epithelial-mesenchymal transition by gemcitabine: Targeting ionizing radiation-induced cellular senescence in lung cancer cell BIOCHEMICAL PHARMACOLOGY Heng Zhou WB Mouse,Human A549 cell,PC-9 cell,Lewis lung carcinoma cell

Metochalcone induces senescence-associated secretory phenotype via JAK2/STAT3 pathway in breast cancer ONCOLOGY RESEARCH JIANBO ZHOU IHC Mouse 1:50 A549 cell-Xenograft,BT549 cell-Xenograft

Homogentisic acid metabolism inhibits papillary thyroid carcinoma proliferation through ROS and p21-induced cell cycle arrest LIFE SCIENCES Ruiwang Xie WB,IHC Human,Mouse 1:1000 IHH4 cell-Xenograft TPC-1 cell, IHH4 cell, K1 cell

CircSSBP2 acts as a MiR-2400 sponge to promote intramuscular preadipocyte proliferation by regulating NDRG1 MOLECULAR GENETICS AND GENOMICS Zhao Yanqing WB Bovine 1:5000 intramuscular preadipocytes

POSTN promotes nucleus pulposus cell senescence and extracellular matrix metabolism via activing Wnt/β-catenin and NF-κB signal pathway in intervertebral disc degeneration CELLULAR SIGNALLING Daxue Zhu WB Human,Rat 1:1000 nucleus pulposus (NP) tissue nucleus pulposus cell

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