Histone H3 (Di Methyl Lys27) Monoclonal Antibody(3B12)

    • Catalog No.:YM3101
    • Applications:WB;IP
    • Reactivity:Human;Mouse;Rat
      • Target:
      • Histone H3
      • Fields:
      • >>Neutrophil extracellular trap formation;>>Alcoholism;>>Shigellosis;>>Transcriptional misregulation in cancer;>>Systemic lupus erythematosus
      • Gene Name:
      • HIST1H3A/HIST1H3B/HIST1H3C/HIST1H3D/HIST1H3E/HIST1H3F/HIST1H3G/HIST1H3H/HIST1H3I/HIST1H3J/HIST2H3A/HIST2H3C/HIST2H3D/H3F3A/H3F3B
      • Protein Name:
      • Histone H3.1/Histone H3.2/Histone H3.3
      • Immunogen:
      • Synthetic Peptide of Histone H3 (Di Methyl Lys27)
      • Specificity:
      • The antibody detects endogenous Histone H3 (di methyl K27) protein.
      • Formulation:
      • PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
      • Source:
      • Monoclonal, Mouse
      • Dilution:
      • WB 1:1000-3000 IP:1:200
      • Purification:
      • The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • H3K27ME2;HIST1H3A;H3FA;HIST1H3B;H3FL;HIST1H3C;H3FC;HIST1H3D;H3FB;HIST1H3E;H3FD;HIST1H3F;H3FI;HIST1H3G;H3FH;HIST1H3H;H3FK;HIST1H3I;H3FF;HIST1H3J;H3FJ;Histone H3.1;Histone H3/a;Histone H3/b;Histone H3/c;Histone H3/d;Histone H3/f;Histone H3/h;Histone H3/i;Histone H3/j;Histone H3/k;Histone H3/l;HIST2H3A;HIST2H3C;H3F2;H3FM;HIST2H3D;Histone H3.2;Histone H3/m;Histone H3/o;H3F3A;H3.3A;H3F3;PP781;H3F3B;H3.3B;Histone H3.3
      • Observed Band(KD):
      • 15kD
      • Background:
      • Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015],
      • Function:
      • caution:Was originally (PubMed:2587222) thought to originate from mouse.,developmental stage:Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.,function:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.,mass spectrometry:Monoisotopic with N-acetylserine PubMed:16457589,miscellaneous:This histone is only present in mammals and is enriched in acetylation of Lys-15 and dimethylation of Lys-10 (H3K9me2).,PTM:Acetylation is generally l
      • Subcellular Location:
      • Nucleus. Chromosome.
      • Expression:
      • Blood,Epithelium,Kidney,Lung,Ovary,Spleen,Uterus,
      • Products Images
      • Wong, Chi Chun, et al. "In Colorectal Cancer Cells With Mutant KRAS, SLC25A22-Mediated Glutaminolysis Reduces DNA Demethylation to Increase WNT Signaling, Stemness, and Drug Resistance." Gastroenterology 159.6 (2020): 2163-2180.
      • Western blot analysis of Hela, diluted at 1) 1:1000 2) 1:3000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
      • 1) Input: Hela Cell Lysate 2) IP product: IP dilute 1:200