His-Tag Monoclonal Antibody(4E6)

    • Catalog No.:YM3004
    • Applications:WB;IP;IF
    • Reactivity:Species independent
      • Target:
      • His-Tag
      • Gene Name:
      • His-Tag
      • Protein Name:
      • His Tag
      • Immunogen:
      • Synthetic peptide:HHHHHH conjugated to KLH.
      • Specificity:
      • The antibody detects C-terminal, internal, and N-terminal His-tag fusion proteins.
      • Formulation:
      • PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
      • Source:
      • Monoclonal, Mouse
      • Dilution:
      • WB 1:3000 IP: 1:200 IF 1:1000
      • Purification:
      • The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Background:
      • A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

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      • Products Images
      • ADAR1 links R-loop homeostasis to ATR activation in replication stress response. NUCLEIC ACIDS RESEARCH Lei Shi WB,CoIP Human HeLa cell
      • Wang, YY., Zhao, B., Wu, MM. et al. Overexpression of neuregulin 1 in GABAergic interneurons results in reversible cortical disinhibition. Nat Commun 12, 278 (2021). 
      • 2ug His fusion protein+ Primary antibody dilution at 1) 1:5000 2) 1:10000
      • IF analysis of 293 cells transfected with a His-tag protein,1:1000 dilution (blue DAPI, red anti-His)
      • IP antibody use:5ug His Mouse IgG1 per ml Lysate,WB 1:3000 1、untransfected 293 cell lysate 2、transfected 293 cell lysate with His-tag fusion protein 3、IP (untransfected 293+anti-His mAb+ Protein G agarose) 4、IP (transfected 293+ normal Mouse IgG+Protein G agarose) 5、IP (transfected 293+anti-His mAb+ Protein G agarose) 6、Recombinant protein (E.coli)