• Target：
• GPI

• Fields：
• >>Glycolysis / Gluconeogenesis;>>Pentose phosphate pathway;>>Starch and sucrose metabolism;>>Amino sugar and nucleotide sugar metabolism;>>Metabolic pathways;>>Carbon metabolism;>>Biosynthesis of nucleotide sugars

• Gene Name：
• GPI

• Protein Name：
• Glucose-6-phosphate isomerase

• Human Gene Id：
• 2821

• Human Swiss Prot No：
• P06744

• Mouse Swiss Prot No：
• P06745

• Immunogen：
• Purified recombinant fragment of human GPI expressed in E. Coli.

• Specificity：
• GPI Monoclonal Antibody detects endogenous levels of GPI protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Monoclonal, Mouse

• Dilution：
• WB 1:500 - 1:2000. IHC 1:200 - 1:1000. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• Affinity purification

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• GPI;Glucose-6-phosphate isomerase;GPI;Autocrine motility factor;AMF;Neuroleukin;NLK;Phosphoglucose isomerase;PGI;Phosphohexose isomerase;PHI;Sperm antigen 36;SA-36

• Molecular Weight(Da)：
• 63kD

• References：
• 1. Biochem Biophys Res Commun. 2004 Oct 15;323(2):518-22.
  2. Biochem Biophys Res Commun. 2006 Oct 20;349(2):838-45.
  3. Hum Mutat. 2006 Nov;27(11):1159.
  4. Leuk Lymphoma. 2006 Oct;47(10):22

• Background：
• This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phosphate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis

• Function：
• catalytic activity:D-glucose 6-phosphate = D-fructose 6-phosphate.,disease:Defects in GPI are a cause of hereditary nonspherocytic hemolytic anemia (HA) [MIM:172400]. Severe GPI deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.,function:Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.,online information:Phosphoglucose isomerase entry,online information:The Singapore human mutation and polymorphism database,pathway:Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.,PTM:Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to s

• Subcellular Location：
• Cytoplasm . Secreted .

• Expression：
• B-cell lymphoma,Brain,Skin,Spleen,Testis,

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