• Target：
• α-protein Kinase 1

• Gene Name：
• ALPK1

• Protein Name：
• Alpha-protein kinase 1

• Human Gene Id：
• 80216

• Human Swiss Prot No：
• Q96QP1

• Mouse Swiss Prot No：
• Q9CXB8

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human ALPK1. AA range:11-60

• Specificity：
• α-protein Kinase 1 Polyclonal Antibody detects endogenous levels of α-protein Kinase 1 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• ALPK1;KIAA1527;LAK;Alpha-protein kinase 1;Chromosome 4 kinase;Lymphocyte alpha-protein kinase

• Observed Band(KD)：
• 139kD

• Background：
• This gene encodes an alpha kinase. Mice which were homozygous for disrupted copies of this gene exhibited coordination defects (PMID: 21208416). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011],

• Function：
• function:Kinases that recognize phosphorylation sites in which the surrounding peptides have an alpha-helical conformation.,similarity:Belongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily.,similarity:Contains 1 alpha-type protein kinase domain.,tissue specificity:Highly expressed in liver.,

• Subcellular Location：
• Cytoplasm, cytosol . Cytoplasm, cytoskeleton, spindle pole . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cell projection, cilium . Localized at the base of primary cilia. .

• Expression：
• Highly expressed in liver. Expressed in the optic nerve and retinal pigmented epithelium. Lower expression is observed in the macula and extramacular retina (PubMed:30967659).

Alpha‐kinase1 promotes tubular injury and interstitial inflammation in diabetic nephropathy by canonical pyroptosis pathway BIOLOGICAL RESEARCH Xuejing Zhu IHC,WB Human,Mouse Renal HK-2 cell

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