α-protein Kinase 1 Polyclonal Antibody

    • Catalog No.:YT5001
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse
      • Target:
      • α-protein Kinase 1
      • Gene Name:
      • ALPK1
      • Protein Name:
      • Alpha-protein kinase 1
      • Human Swiss Prot No:
      • Q96QP1
      • Mouse Swiss Prot No:
      • Q9CXB8
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human ALPK1. AA range:11-60
      • Specificity:
      • α-protein Kinase 1 Polyclonal Antibody detects endogenous levels of α-protein Kinase 1 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • ALPK1;KIAA1527;LAK;Alpha-protein kinase 1;Chromosome 4 kinase;Lymphocyte alpha-protein kinase
      • Observed Band(KD):
      • 139kD
      • Background:
      • This gene encodes an alpha kinase. Mice which were homozygous for disrupted copies of this gene exhibited coordination defects (PMID: 21208416). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011],
      • Function:
      • function:Kinases that recognize phosphorylation sites in which the surrounding peptides have an alpha-helical conformation.,similarity:Belongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily.,similarity:Contains 1 alpha-type protein kinase domain.,tissue specificity:Highly expressed in liver.,
      • Subcellular Location:
      • Cytoplasm, cytosol . Cytoplasm, cytoskeleton, spindle pole . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cell projection, cilium . Localized at the base of primary cilia. .
      • Expression:
      • Highly expressed in liver. Expressed in the optic nerve and retinal pigmented epithelium. Lower expression is observed in the macula and extramacular retina (PubMed:30967659).
      • Products Images
      • Western Blot analysis of various cells using α-protein Kinase 1 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
      • Western blot analysis of lysates from COLO and HepG2 cells, using ALPK1 Antibody. The lane on the right is blocked with the synthesized peptide.
      • Western blot analysis of the lysates from 293 cells using ALPK1 antibody.
      • Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).