Wnt-1 Polyclonal Antibody

    • Catalog No.:YT4907
    • Applications:WB,IHC-p,IF,ELISA
    • Reactivity:Human,Mouse
      • Gene Name:
      • WNT1
      • Protein Name:
      • Proto-oncogene Wnt-1
      • Human Gene Id:
      • 7471
      • Human Swiss Prot No:
      • P04628
      • Mouse Swiss Prot No:
      • P04426
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human WNT1. AA range:301-350
      • Specificity:
      • Wnt-1 Polyclonal Antibody detects endogenous levels of Wnt-1 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • WNT1; INT1; Proto-oncogene Wnt-1; Proto-oncogene Int-1 homolog
      • MolecularWeight(Da):
      • 40982
      • Observed Band(KD):
      • 45
      • Background:
      • Wnt family member 1(WNT1) Homo sapiens The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is very conserved in evolution, and the protein encoded by this gene is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. This gene is clustered with another family member, WNT10B, in
      • Function:
      • function:Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters.,similarity:Belongs to the Wnt family.,subunit:Interacts with PORCN. Interacts with RSPO1, RSPO2 and RSPO3.,
      • Subcellular Location:
      • extracellular region,proteinaceous extracellular matrix,extracellular space,cytoplasm,endoplasmic reticulum lumen,Golgi lumen,plasma membrane,cell surface,endocytic vesicle membrane,extracellular exosome,
      • Expression:
      • Testis,
      • Products Images
      • Western Blot analysis of various cells using Wnt-1 Polyclonal Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
      • Western Blot analysis of HT29 cells using Wnt-1 Polyclonal Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
      • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using WNT1 Antibody. The picture on the right is blocked with the synthesized peptide.