• Target：
• Topo IIβ

• Fields：
• >>Platinum drug resistance

• Gene Name：
• TOP2B

• Protein Name：
• DNA topoisomerase 2-beta

• Human Gene Id：
• 7155

• Human Swiss Prot No：
• Q02880

• Mouse Gene Id：
• 21974

• Mouse Swiss Prot No：
• Q64511

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human TOP2B. AA range:1-50

• Specificity：
• Topo IIβ Polyclonal Antibody detects endogenous levels of Topo IIβ protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• TOP2B;DNA topoisomerase 2-beta;DNA topoisomerase II; beta isozyme

• Observed Band(KD)：
• 183kD

• Background：
• This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also pla

• Function：
• catalytic activity:ATP-dependent breakage, passage and rejoining of double-stranded DNA.,function:Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.,function:Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Indirectly ivolved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.,miscellaneous:Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs

• Subcellular Location：
• Nucleus, nucleolus . Nucleus, nucleoplasm . Nucleus .

• Expression：
• Expressed in the tonsil, spleen, lymph node, thymus, skin, pancreas, testis, colon, kidney, liver, brain and lung (PubMed:9155056). Also found in breast, colon and lung carcinomas, Hodgkin's disease, large-cell non-Hodgkin's lymphoma, lymphocytic lymphomas and seminomas (PubMed:9155056).

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