TEF-4 Polyclonal Antibody

    • Catalog No.:YT4597
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse
      • Target:
      • TEF-4
      • Fields:
      • >>Hippo signaling pathway;>>Hippo signaling pathway - multiple species
      • Gene Name:
      • TEAD2
      • Protein Name:
      • Transcriptional enhancer factor TEF-4
      • Human Gene Id:
      • 8463
      • Human Swiss Prot No:
      • Q15562
      • Mouse Swiss Prot No:
      • P48301
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human TEAD2. AA range:71-120
      • Specificity:
      • TEF-4 Polyclonal Antibody detects endogenous levels of TEF-4 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500-2000 IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • TEAD2;TEF4;Transcriptional enhancer factor TEF-4;TEA domain family member 2;TEAD-2
      • Molecular Weight(Da):
      • 49kD
      • Background:
      • function:Putative transcription factor that binds to the SPH and GT-IIC "enhansons" (5'-GTGGAATGT-3'). May be involved in the gene regulation of neural development. Binds to the M-CAT motif.,similarity:Contains 1 TEA DNA-binding domain.,
      • Function:
      • function:Putative transcription factor that binds to the SPH and GT-IIC "enhansons" (5'-GTGGAATGT-3'). May be involved in the gene regulation of neural development. Binds to the M-CAT motif.,similarity:Contains 1 TEA DNA-binding domain.,
      • Subcellular Location:
      • Nucleus.
      • Expression:
      • Brain,Eye,Skin,Testis,
      • Products Images
      • Immunofluorescence analysis of Hela cell. 1,TEF-4 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). β-actin Monoclonal Antibody(5B7)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).
      • Western Blot analysis of mouse-brain cells using TEF-4 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
      • Immunofluorescence analysis of HepG2 cells, using TEAD2 Antibody. The picture on the right is blocked with the synthesized peptide.
      • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using TEAD2 Antibody. The picture on the right is blocked with the synthesized peptide.