SRp75 Polyclonal Antibody

    • Catalog No.:YT4421
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse
      • Target:
      • SRp75
      • Fields:
      • >>Spliceosome;>>Herpes simplex virus 1 infection
      • Gene Name:
      • SRSF4
      • Protein Name:
      • Serine/arginine-rich splicing factor 4
      • Human Gene Id:
      • 6429
      • Human Swiss Prot No:
      • Q08170
      • Mouse Swiss Prot No:
      • Q8VE97
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human SFRS4. AA range:111-160
      • Specificity:
      • SRp75 Polyclonal Antibody detects endogenous levels of SRp75 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • SRSF4;SFRS4;SRP75;Serine/arginine-rich splicing factor 4;Pre-mRNA-splicing factor SRP75;SRP001LB;Splicing factor; arginine/serine-rich 4
      • Observed Band(KD):
      • 57kD
      • Background:
      • This gene encodes a member of the arginine/serine-rich splicing factor family. The encoded protein likely functions in mRNA processing. [provided by RefSeq, Feb 2009],
      • Function:
      • function:A probable role in alternative splice site selection during pre-mRNA splicing.,PTM:Extensively phosphorylated on serine residues in the RS domain.,similarity:Belongs to the splicing factor SR family.,similarity:Contains 2 RRM (RNA recognition motif) domains.,subunit:Found in a pre-mRNA splicing complex with SFRS4, SFRS5, SNRNP70, SNRPA1, SRRM1 and SRRM2. Interacts with PNN.,
      • Subcellular Location:
      • Nucleus speckle .
      • Expression:
      • Aorta endothelial cell,Cerebrum,Epithelium,Lymph,
      • Products Images
      • Western Blot analysis of various cells using SRp75 Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
      • Immunohistochemical analysis of paraffin-embedded Human thyroid gland. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
      • Western blot analysis of lysates from LOVO cells, using SFRS4 Antibody. The lane on the right is blocked with the synthesized peptide.