RIN3 Polyclonal Antibody

    • Catalog No.:YT4145
    • Applications:WB;ELISA;IHC
    • Reactivity:Human;Rat;Mouse;
      • Target:
      • RIN3
      • Gene Name:
      • RIN3
      • Protein Name:
      • Ras and Rab interactor 3
      • Human Swiss Prot No:
      • Q8TB24
      • Mouse Swiss Prot No:
      • P59729
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human RIN3. AA range:391-440
      • Specificity:
      • RIN3 Polyclonal Antibody detects endogenous levels of RIN3 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • RIN3;Ras and Rab interactor 3;Ras interaction/interference protein 3
      • Observed Band(KD):
      • 107kD
      • Background:
      • Summary: This protein encoded by this gene is a member of the RIN family of Ras interaction-interference proteins, which are binding partners to the RAB5 small GTPases. The protein functions as a guanine nucleotide exchange for RAB5B and RAB31. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016],
      • Function:
      • caution:The region from Asp-168 to Pro-327 is deduced from the genomic sequence by similarity to the mouse sequence.,function:Potential Ras effector protein. May function as a guanine nucleotide exchange (GEF), by exchanging bound GDP for free GTP.,similarity:Belongs to the RIN (Ras interaction/interference) family.,similarity:Contains 1 Ras-associating domain.,similarity:Contains 1 SH2 domain.,similarity:Contains 1 VPS9 domain.,subcellular location:Activation of tyrosine phosphorylation signaling induces translocation to cytoplasmic vesicles.,subunit:Interacts with RAB5B and BIN1.,tissue specificity:Widely expressed.,
      • Subcellular Location:
      • Cytoplasm . Cytoplasmic vesicle . Early endosome . Activation of tyrosine phosphorylation signaling induces translocation to cytoplasmic vesicles. .
      • Expression:
      • Widely expressed.
      • Products Images
      • Western Blot analysis of various cells using RIN3 Polyclonal Antibody
      • Western blot analysis of lysates from HUVEC and K562 cells, using RIN3 Antibody. The lane on the right is blocked with the synthesized peptide.
      • Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).