Pitx1 Polyclonal Antibody
- Catalog No.:YT3740
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse;Rat
- Target:
- Pitx1
- Gene Name:
- PITX1
- Protein Name:
- Pituitary homeobox 1
- Human Gene Id:
- 5307
- Human Swiss Prot No:
- P78337
- Mouse Gene Id:
- 18740
- Mouse Swiss Prot No:
- P70314
- Rat Gene Id:
- 113983
- Rat Swiss Prot No:
- Q99NA7
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human PITX1. AA range:121-170
- Specificity:
- Pitx1 Polyclonal Antibody detects endogenous levels of Pitx1 protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- PITX1;BFT;PTX1;Pituitary homeobox 1;Hindlimb-expressed homeobox protein backfoot;Homeobox protein PITX1;Paired-like homeodomain transcription factor 1
- Observed Band(KD):
- 34kD
- Background:
- This gene encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. Members of this family are involved in organ development and left-right asymmetry. This protein acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin. [provided by RefSeq, Jul 2008],
- Function:
- function:May play a role in the development of anterior structures, and in particular, the brain and facies and in specifying the identity or structure of hindlimb.,similarity:Belongs to the paired homeobox family. Bicoid subfamily.,similarity:Contains 1 homeobox DNA-binding domain.,similarity:Contains 1 OAR domain.,
- Subcellular Location:
- Nucleus.
- Expression:
- Embryo,Epithelium,Pancreas,Placenta,
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- May 20-2022
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- July 13-2018
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- July 13-2018
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- July 13-2018
- Antibody-FAQs
- Products Images
- Western Blot analysis of various cells using Pitx1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Western Blot analysis of Jurkat cells using Pitx1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Western blot analysis of lysates from Jurkat and COLO cells, using PITX1 Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human spleen. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).