FKHR (Phospho Ser249) rabbit pAb

    • Catalog No.:YP1671
    • Applications:WB
    • Reactivity:Human;Mouse;Rat
      • Target:
      • FoxO1
      • Fields:
      • >>FoxO signaling pathway;>>AMPK signaling pathway;>>Longevity regulating pathway;>>Longevity regulating pathway - multiple species;>>Cellular senescence;>>Insulin signaling pathway;>>Thyroid hormone signaling pathway;>>Glucagon signaling pathway;>>Insulin resistance;>>AGE-RAGE signaling pathway in diabetic complications;>>Alcoholic liver disease;>>Shigellosis;>>Human papillomavirus infection;>>Pathways in cancer;>>Transcriptional misregulation in cancer;>>Prostate cancer
      • Gene Name:
      • FOXO1 FKHR FOXO1A
      • Protein Name:
      • FKHR (Phospho-Ser249)
      • Human Gene Id:
      • 2308
      • Human Swiss Prot No:
      • Q12778
      • Mouse Swiss Prot No:
      • Q9R1E0
      • Immunogen:
      • Synthesized peptide derived from human FKHR (Phospho-Ser249)
      • Specificity:
      • This antibody detects endogenous levels of FKHR (Phospho-Ser249) at Human, Mouse,Rat
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500-2000
      • Purification:
      • The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • Forkhead box protein O1 (Forkhead box protein O1A) (Forkhead in rhabdomyosarcoma)
      • Molecular Weight(Da):
      • 72kD
      • Background:
      • This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008],
      • Function:
      • disease:Chromosomal aberrations involving FOXO1 are a cause of rhabdomyosarcoma 2 (RMS2) [MIM:268220]; also known as alveolar rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator.,function:Transcription factor.,PTM:Phosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 fork-head DNA-binding domain.,subcellular location:Shuttles betw
      • Subcellular Location:
      • Cytoplasm . Nucleus . Shuttles between the cytoplasm and nucleus. Largely nuclear in unstimulated cells (PubMed:11311120, PubMed:12228231, PubMed:19221179, PubMed:21245099, PubMed:20543840, PubMed:25009184). In osteoblasts, colocalizes with ATF4 and RUNX2 in the nucleus (By similarity). Serum deprivation increases localization to the nucleus, leading to activate expression of SOX9 and subsequent chondrogenesis (By similarity). Insulin-induced phosphorylation at Ser-256 by PKB/AKT1 leads, via stimulation of Thr-24 phosphorylation, to binding of 14-3-3 proteins and nuclear export to the cytoplasm where it is degraded by the ubiquitin-proteosomal pathway (PubMed:11237865, PubMed:12228231). Phosphorylation at Ser-249 by CDK1 disrupts binding of 14-3-3 proteins and promotes nuclear accumulation
      • Expression:
      • Ubiquitous.
      • Products Images
      • Western Blot analysis of HepG2 mouse brain tissue, rat brain tissue, using primary antibody at 1:1000 dilution 4°C, overnight. Secondary antibody(catalog#:RS23920) was diluted at 1:10000 25°C,1.5hours