Wee 1 (phospho Ser53) Polyclonal Antibody

    • Catalog No.:YP1064
    • Applications:IHC-p,ELISA
    • Reactivity:Human,Mouse,Rat
      • Gene Name:
      • WEE1
      • Protein Name:
      • Wee1-like protein kinase
      • Human Gene Id:
      • 7465
      • Human Swiss Prot No:
      • P30291
      • Mouse Swiss Prot No:
      • P47810
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human WEE1 around the phosphorylation site of Ser53. AA range:19-68
      • Specificity:
      • Phospho-Wee 1 (S53) Polyclonal Antibody detects endogenous levels of Wee 1 protein only when phosphorylated at S53.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • WEE1; Wee1-like protein kinase; WEE1hu; Wee1A kinase
      • MolecularWeight(Da):
      • 71597
      • Background:
      • WEE1 G2 checkpoint kinase(WEE1) Homo sapiens This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul 2008],
      • Function:
      • catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,cofactor:Binds 2 magnesium ions per subunit.,enzyme regulation:Synthesis is increased during S and G2 phases, presumably by an increase in transcription; activity is decreased by phosphorylation during m phase. Protein levels fall in M phase as a result of decreased synthesis combined with degradation. Activity seems to be negatively regulated by phosphorylation upon entry into mitosis, although N-terminal phosphorylation might also regulate the protein stability via protection from proteolysis or might regulate the subcellular location.,function:May act as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDC2 before the onset of mitosis. Its activity increases during S and G2 phases and decreases at M phase
      • Subcellular Location:
      • nucleus,nucleoplasm,nucleolus,cytoplasm,
      • Expression:
      • Amygdala,Blood,Epithelium,Human uterus endothel primary cell culture,Placenta,Skin,
      • Products Images
      • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using WEE1 (Phospho-Ser53) Antibody
      • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using WEE1 (Phospho-Ser53) Antibody. The picture on the right is blocked with the phospho peptide.