Total WEE1 Cell-Based Colorimetric ELISA Kit

  • Catalog No.：KA4324C
  • Applications：ELISA
  • Reactivity：Human;Mouse;Rat
    • Gene Name：
    • WEE1
    • Human Gene Id：
    • 7465
    • Human Swiss Prot No：
    • P30291
    • Mouse Swiss Prot No：
    • P47810
    • Storage Stability：
    • 2-8°C/6 months
    • Other Name：
    • Wee1-like protein kinase (WEE1hu) (EC 2.7.10.2) (Wee1A kinase)
    • Detection Method：
    • Colorimetric
    • Background：
    • catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,cofactor:Binds 2 magnesium ions per subunit.,enzyme regulation:Synthesis is increased during S and G2 phases, presumably by an increase in transcription; activity is decreased by phosphorylation during m phase. Protein levels fall in M phase as a result of decreased synthesis combined with degradation. Activity seems to be negatively regulated by phosphorylation upon entry into mitosis, although N-terminal phosphorylation might also regulate the protein stability via protection from proteolysis or might regulate the subcellular location.,function:May act as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDC2 before the onset of mitosis. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. Specifically phosphorylates and inactivates cyclin B1-complexed CDC2 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDC2 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDC2 does not occur.,PTM:Phosphorylated during M and G1 phases. Also autophosphorylated.,PTM:Ubiquitinated and degraded at the onset of G2/M phase.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.,similarity:Contains 1 protein kinase domain.,
    • Function：
    • M phase of mitotic cell cycle, mitotic cell cycle, M phase, nuclear division, protein amino acid phosphorylation,phosphorus metabolic process, phosphate metabolic process, cell cycle, mitosis, phosphorylation, cell cycle process,cell cycle phase, organelle fission, cell division,
    • Subcellular Location：
    • Nucleus.

    Attributes

    • Code：
    • KA4324C
    • Size
    • 96 / well
    • Price$：
    • 330
    • Delivery：
    • 2024-12-07
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