SPHK2 Mouse mAb(7A5)
- Catalog No.:YM3806
- Applications:WB;IHC
- Reactivity:Human;Mouse;Rat
- Target:
- SphK2
- Gene Name:
- SPHK2
- Protein Name:
- SPHK2
- Human Gene Id:
- 56848
- Human Swiss Prot No:
- Q9NRA0
- Mouse Gene Id:
- 56632
- Mouse Swiss Prot No:
- Q9JIA7
- Immunogen:
- Synthesized peptide derived from human SPHK2
- Specificity:
- This antibody detects endogenous levels of SPHK2 at Human, Mouse,Rat
- Formulation:
- Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
- Source:
- Mouse,monoclonal
- Dilution:
- WB 1:500-2000 IHC 1:50-200
- Purification:
- The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- Sphingosine kinase 2 (SK 2) (SPK 2) (EC 2.7.1.91)
- Molecular Weight(Da):
- 72kD
- Function:
- alternative products:Experimental confirmation may be lacking for some isoforms,catalytic activity:ATP + sphinganine = ADP + sphinganine 1-phosphate.,catalytic activity:ATP + sphingosine = ADP + sphingosine 1-phosphate.,cofactor:Magnesium.,function:Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra-and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine.,similarity:Contains 1 DAGKc domain.,
- Subcellular Location:
- Cytoplasm . Nucleus . Endoplasmic reticulum . Mitochondrion inner membrane . In nucleus, located in nucleosomes where it associates with core histone proteins such as histone 3 (PubMed:19729656). In brains of patients with Alzheimer's disease, may be preferentially localized in the nucleus. Cytosolic expression decrease correlates with the density of amyloid deposits (PubMed:29615132). In apoptotic cells, colocalizes with CASP1 in cell membrane where is cleaved and released from cells in an active form (PubMed:20197547). .; [Isoform 2]: Lysosome membrane .
- Expression:
- Mainly expressed in adult kidney, liver, and brain (PubMed:10751414). Expressed in cerebral cortex and hippocampus (at protein level) (PubMed:29615132). Isoform 1 is the predominant form expressed in most tissues (PubMed:16103110).
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