Villin Mouse mAb(2C9)

    • Catalog No.:YM3801
    • Applications:WB;IHC
    • Reactivity:Human;Mouse;Rat
      • Target:
      • Villin
      • Gene Name:
      • VIL1 VIL
      • Protein Name:
      • Villin
      • Human Gene Id:
      • 7429
      • Human Swiss Prot No:
      • P09327
      • Mouse Swiss Prot No:
      • Q62468
      • Immunogen:
      • Synthesized peptide derived from human Villin
      • Specificity:
      • This antibody detects endogenous levels of Villin at Human, Mouse,Rat
      • Formulation:
      • Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
      • Source:
      • Mouse,monoclonal
      • Dilution:
      • WB 1:500-2000 IHC 1:50-200
      • Purification:
      • The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • Villin-1
      • Observed Band(KD):
      • 93kD
      • Function:
      • domain:Consists of a large core fragment, the N-terminal portion, and a small headpiece, the C-terminal portion. The headpiece binds F-actin strongly in both the presence and absence of calcium.,function:Ca(2+)-regulated actin-binding protein.,similarity:Belongs to the villin/gelsolin family.,similarity:Contains 1 HP (headpiece) domain.,similarity:Contains 6 gelsolin-like repeats.,subunit:Monomer.,tissue specificity:Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells.,
      • Subcellular Location:
      • Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Cell projection, ruffle. Cell projection, microvillus. Cell projection, filopodium tip . Cell projection, filopodium . Relocalized in the tip of cellular protrusions and filipodial extensions upon infection with S.flexneri in primary intestinal epithelial cells (IEC) and in the tail-like structures forming the actin comets of S.flexneri. Redistributed to the leading edge of hepatocyte growth factor (HGF)-induced lamellipodia (By similarity). Rapidly redistributed to ruffles and lamellipodia structures in response to autotaxin, lysophosphatidic acid (LPA) and epidermal growth factor (EGF) treatment. .
      • Expression:
      • Specifically expressed in epithelial cells. Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells. Expressed in canalicular microvilli of hepatocytes (at protein level).
      • Products Images
      • Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1,primary Antibody was diluted at 1:200(4° overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200