• Target：
• Villin

• Gene Name：
• VIL1 VIL

• Protein Name：
• Villin-1

• Human Gene Id：
• 7429

• Human Swiss Prot No：
• P09327

• Immunogen：
• Synthesized peptide derived from human Villin

• Specificity：
• This antibody detects endogenous levels of human Villin. Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH8.0 was highly recommended as antigen repair method in paraffin section

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Monoclonal, Mouse/IgG2b, Kappa

• Dilution：
• IHC-p 1:100-500，WB 1:500-2000

• Purification：
• The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

• Storage Stability：
• -20°C/1 year

• Background：
• This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. [provided by RefSeq, Jul 2008],

• Function：
• domain:Consists of a large core fragment, the N-terminal portion, and a small headpiece, the C-terminal portion. The headpiece binds F-actin strongly in both the presence and absence of calcium.,function:Ca(2+)-regulated actin-binding protein.,similarity:Belongs to the villin/gelsolin family.,similarity:Contains 1 HP (headpiece) domain.,similarity:Contains 6 gelsolin-like repeats.,subunit:Monomer.,tissue specificity:Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells.,

• Subcellular Location：
• Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Cell projection, ruffle. Cell projection, microvillus. Cell projection, filopodium tip . Cell projection, filopodium . Relocalized in the tip of cellular protrusions and filipodial extensions upon infection with S.flexneri in primary intestinal epithelial cells (IEC) and in the tail-like structures forming the actin comets of S.flexneri. Redistributed to the leading edge of hepatocyte growth factor (HGF)-induced lamellipodia (By similarity). Rapidly redistributed to ruffles and lamellipodia structures in response to autotaxin, lysophosphatidic acid (LPA) and epidermal growth factor (EGF) treatment. .

• Expression：
• Specifically expressed in epithelial cells. Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells. Expressed in canalicular microvilli of hepatocytes (at protein level).

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