Villin (PT0254) mouse mAb

    • Catalog No.:YM6222
    • Applications:IHC,WB
    • Reactivity:Human;Mouse
      • Gene Name:
      • VIL1 VIL
      • Protein Name:
      • Villin-1
      • Human Gene Id:
      • 7429
      • Human Swiss Prot No:
      • P09327
      • Immunogen:
      • Synthesized peptide derived from human Villin
      • Specificity:
      • This antibody detects endogenous levels of human Villin. Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH8.0 was highly recommended as antigen repair method in paraffin section
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Monoclonal, Mouse/IgG2b, Kappa
      • Dilution:
      • IHC-p 1:100-500,WB 1:500-2000
      • Purification:
      • The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
      • Concentration:
      • 0.3mg/mL
      • Storage Stability:
      • -20°C/1 year
      • Background:
      • This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. [provided by RefSeq, Jul 2008],
      • Function:
      • domain:Consists of a large core fragment, the N-terminal portion, and a small headpiece, the C-terminal portion. The headpiece binds F-actin strongly in both the presence and absence of calcium.,function:Ca(2+)-regulated actin-binding protein.,similarity:Belongs to the villin/gelsolin family.,similarity:Contains 1 HP (headpiece) domain.,similarity:Contains 6 gelsolin-like repeats.,subunit:Monomer.,tissue specificity:Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells.,
      • Subcellular Location:
      • Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Cell projection, ruffle. Cell projection, microvillus. Cell projection, filopodium tip . Cell projection, filopodium . Relocalized in the tip of cellular protrusions and filipodial extensions upon infection with S.flexneri in primary intestinal epithelial cells (IEC) and in the tail-like structures forming the actin comets of S.flexneri. Redistributed to the leading edge of hepatocyte growth factor (HGF)-induced lamellipodia (By similarity). Rapidly redistributed to ruffles and lamellipodia structures in response to autotaxin, lysophosphatidic acid (LPA) and epidermal growth factor (EGF) treatment. .
      • Expression:
      • Specifically expressed in epithelial cells. Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells. Expressed in canalicular microvilli of hepatocytes (at protein level).
      • Products Images
      • Immunohistochemical analysis of paraffin-embedded Colon. 1, Antibody was diluted at 1:200(4° overnight). 2, TRIS-EDTA of pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
      • Immunohistochemical analysis of paraffin-embedded human stomach Antibody was diluted at 1:200(4° overnight).
      • Immunohistochemical analysis of paraffin-embedded human rectal cancer Antibody was diluted at 1:200(4° overnight).
      • Immunohistochemical analysis of paraffin-embedded human stomach Antibody was diluted at 1:200(4° overnight).
      • Immunohistochemical analysis of paraffin-embedded human stomach Antibody was diluted at 1:200(4° overnight).
      • Human appendix tissue was stained with Anti-Villin (ABT097) Antibody
      • Human kidney tissue was stained with Anti-Villin (ABT097) Antibody
      • Human colon tissue was stained with Anti-Villin (ABT097) Antibody
      • Human colon carcinoma tissue was stained with Anti-Villin (ABT097) Antibody
      • Human small intestine tissue was stained with Anti-Villin (ABT097) Antibody
      • Various whole cell lysates were separated by 8% SDS-PAGE, and the membrane was blotted with anti-Villin antibody. The HRP-conjugated anti-Mouse IgG antibody was used to detect the antibody. Predicted band size: 93 kDa
      • Whole cell lysates of HepG2 were separated by 8% SDS-PAGE, and the membrane was blotted with anti-Villin antibody. The HRP-conjugated anti-Mouse IgG antibody was used to detect the antibody. Predicted band size: 93 kDa