• Target：
• HMGB1

• Fields：
• >>Base excision repair;>>Autophagy - animal;>>Necroptosis;>>Neutrophil extracellular trap formation

• Gene Name：
• HMGB1 HMG1

• Protein Name：
• HMG-1 (Acetyl Lys3)

• Human Gene Id：
• 3146

• Human Swiss Prot No：
• P09429

• Mouse Gene Id：
• 100862258

• Mouse Swiss Prot No：
• P63158

• Rat Gene Id：
• 25459

• Rat Swiss Prot No：
• P63159

• Immunogen：
• Synthesized peptide derived from human HMG-1 (Acetyl Lys3)

• Specificity：
• This antibody detects endogenous levels of Human,Mouse,Rat HMG-1 (Acetyl Lys3)

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:1000-2000 ELISA 1:5000-20000

• Purification：
• The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• High mobility group protein B1 (High mobility group protein 1;HMG-1)

• Observed Band(KD)：
• about 30kd

• Background：
• function:Binds preferentially single-stranded DNA and unwinds double stranded DNA.,similarity:Belongs to the HMGB family.,similarity:Contains 2 HMG box DNA-binding domains.,

• Function：
• negative regulation of transcription from RNA polymerase II promoter, DNA metabolic process, DNA replication, DNA-dependent DNA replication, DNA ligation, DNA unwinding during replication, DNA repair, base-excision repair, base-excision repair, DNA ligation, DNA recombination, chromatin organization, regulation of transcription, DNA-dependent,regulation of transcription from RNA polymerase II promoter, anti-apoptosis, response to DNA damage stimulus,negative regulation of biosynthetic process, negative regulation of macromolecule biosynthetic process, negative regulation of macromolecule metabolic process, negative regulation of gene expression, regulation of cell death,negative regulation of transcription, negative regulation of transcriptional preinitiation complex assembly, negative regulation of cellular biosynthetic process, DNA geometric change, DNA duplex unwinding, cellular respo

• Subcellular Location：
• Nucleus . Chromosome . Cytoplasm . Secreted . Cell membrane ; Peripheral membrane protein ; Extracellular side . Endosome . Endoplasmic reticulum-Golgi intermediate compartment . In basal state predominantly nuclear. Shuttles between the cytoplasm and the nucleus (PubMed:12231511, PubMed:17114460). Translocates from the nucleus to the cytoplasm upon autophagy stimulation (PubMed:20819940). Release from macrophages in the extracellular milieu requires the activation of NLRC4 or NLRP3 inflammasomes (By similarity). Passively released to the extracellular milieu from necrotic cells by diffusion, involving the fully reduced HGMB1 which subsequently gets oxidized (PubMed:19811284). Also released from apoptotic cells (PubMed:16855214, PubMed:18631454). Active secretion from a variety of immune a

• Expression：
• Ubiquitous. Expressed in platelets (PubMed:11154118).

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