S100 (ABT094) IHC kit_ImmunoWay Biotechnology Company |抗体|病理抗体|诊断抗体

S100 (ABT094) IHC kit

Specificity：
The antibody can specifically recognize human S100B protein, and has a weak reaction with S100A1, but no cross reaction with S100A4 or S100A6.

Purification：
The antibody was affinity-purified from ascites by affinity-chromatography using specific immunogen.

Other Name：
Protein S100-B (S-100 protein beta chain;S-100 protein subunit beta;S100 calcium-binding protein B)

Background：
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy

Function：
function:Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase.,miscellaneous:In addition to metal-ion binding, this protein is involved with the regulation of protein phosphorylation in brain tissue.,similarity:Belongs to the S-101 family.,similarity:Contains 2 EF-hand domains.,subunit:Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. The S100B dimer binds two molecules of STK38 (By similarity). The S100B dimer interacts with two molecules of CAPZA1.,tissue specificity:Although predominant among the wa

Expression：
Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.

Fluorescence multiplex immunohistochemical analysis of Mouse brain tissue (formalin-fixed paraffin-embedded section). The immunostaining was performed by Sextuple-Fluorescence kit (RS0039, Immunoway). GFAP mouse mAb(YM4426 Immunoway) green, S100 mouse mAb(YM6987 Immunoway) cyan,Neurofilament mouse mAb(YM6897 Immunoway) purple,Iba 1 mouse mAb(YM4765 Immunoway) red,CD8 a mouse mAb(YM4815 Immunoway) yellow, The section was incubated in 5 rounds of staining; sequentially for Anti-antibodies; each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Immunoway YS0004, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual dyes was performed using a Slideviewer Imaging System (Excilone).