• Target：
• PDK1

• Fields：
• >>HIF-1 signaling pathway;>>Central carbon metabolism in cancer

• Gene Name：
• PDK1

• Protein Name：
• [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1 mitochondrial

• Human Gene Id：
• 5163

• Human Swiss Prot No：
• Q15118

• Mouse Swiss Prot No：
• Q8BFP9

• Rat Swiss Prot No：
• Q63065

• Immunogen：
• Purified recombinant fragment of human PDK1 expressed in E. Coli.

• Specificity：
• PDK1 Monoclonal Antibody detects endogenous levels of PDK1 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Monoclonal, Mouse

• Dilution：
• WB 1:500 - 1:2000. IHC 1:200 - 1:1000. IF 1:200 - 1:1000. Flow cytometry: 1:200 - 1:400. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• Affinity purification

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• PDK1;PDHK1;[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1; mitochondrial;Pyruvate dehydrogenase kinase isoform 1

• Molecular Weight(Da)：
• 49kD

• References：
• 1. Nat Cell Biol. 2008 Feb;10(2):127-37.
  2. Blood. 2008 Apr 1;111(7):3723-34.
  3. J Biol Chem. 2007 Apr 20;282(16):12272-89.

• Background：
• Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2013],

• Function：
• catalytic activity:ATP + [pyruvate dehydrogenase (acetyl-transferring)] = ADP + [pyruvate dehydrogenase (acetyl-transferring)] phosphate.,function:Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.,similarity:Belongs to the PDK/BCKDK protein kinase family.,similarity:Contains 1 histidine kinase domain.,tissue specificity:Expressed predominantly in the heart.,

• Subcellular Location：
• Mitochondrion matrix .

• Expression：
• Expressed predominantly in the heart. Detected at lower levels in liver, skeletal muscle and pancreas.

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