• Target：
• GSDMD

• Fields：
• >>Neutrophil extracellular trap formation;>>NOD-like receptor signaling pathway;>>Salmonella infection

• Gene Name：
• GSDMD

• Protein Name：
• Gasdermin domain-containing protein 1,Gasdermin-D,GSDMD,GSDMD-CT,GSDMD-NT,GSDMD_HUMAN

• Human Swiss Prot No：
• P57764

• Immunogen：
• Synthesized peptide derived from human GSDMD. AA range:1-100

• Specificity：
• The antibody detects endogenous full lenth and n-ternal fragment of gsdmd protein,

• Formulation：
• Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• IF 1:50-200 WB 1:1000-3000 IHC 1:50-300

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Observed Band(KD)：
• 36 55kD

• Function：
• Cleavage at Asp-275 by CASP1 (mature and uncleaved precursor forms), CASP4, CASP5 or CASP8 relieves autoinhibition and is sufficient to initiate pyroptosis (PubMed:26375003, PubMed:29898893, PubMed:32109412). Cleavage by CASP1 and CASP4 is not strictly dependent on the consensus cleavage site on GSDMD but depends on an exosite interface on CASP1 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part (PubMed:32109412). Cleavage by CASP8 takes place following inactivation of MAP3K7/TAK1 by Yersinia toxin YopJ (By similarity). Cleavage at Asp-87 by CASP3 or CAPS7 inactivates the ability to mediate pyroptosis (PubMed:28392147, PubMed:28045099).

• Subcellular Location：
• [Gasdermin-D]: Cytoplasm, cytosol . Inflammasome . In response to a canonical inflammasome stimulus, such as nigericin, recruited to NLRP3 inflammasone with similar kinetics to that of uncleaved CASP1 precursor. .; [Gasdermin-D, N-terminal]: Cell membrane ; Multi-pass membrane protein . Secreted . Released in the extracellular milieu following pyroptosis. .; [Gasdermin-D, C-terminal]: Cytoplasm, cytosol .

• Expression：
• Expressed in the suprabasal cells of esophagus, as well as in the isthmus/neck, pit, and gland of the stomach, suggesting preferential expression in differentiating cells.

Coxsackievirus Group B3 Has Oncolytic Activity against Colon Cancer through Gasdermin E-Mediated Pyroptosis Cancers Yejia Zhang, Tian Xu, Huizhen Tian, Jianfeng Wu, Xiaomin Yu, Lingbing Zeng, Fadi Liu, Qiong Liu, Xiaotian Huang WB Human HT-29 cell, HCT-116 cell

Astaxanthin Prevents Tuberculosis-Associated Inflammatory Injury by Inhibiting the Caspase 4/11-Gasdermin-Pyroptosis Pathway Evidence-based Complementary and Alternative Medicine Li Li, Zulipikaer Abudureheman, Xuemei Zhong, Liang Gao, Hui Gong, Chuanjiang He, Boyi Yang, Jie Ren, Ayiguli Alimu, Subinuer Yilamujiang, Fan Yang, Xiaoguang Zou WB Mouse MLE-12 cell

Reduced hydrogen sulfide production contributes to adrenal insufficiency induced by hypoxia via modulation of NLRP3 inflammasome activation REDOX REPORT Changnan Wang WB Mouse adrenal glands

IL-37 alleviates TNF-α-induced pyroptosis of rheumatoid arthritis fibroblast-like synoviocytes by inhibiting the NF-κB/GSDMD signaling pathway IMMUNOBIOLOGY Qiang Shu IHC Human synovial tissue

Hypoxic preconditioned mesenchymal stem cells ameliorate rat brain injury after cardiopulmonary resuscitation by suppressing neuronal pyroptosis. Feng Chen WB Rat cortical tissue

Targeting the mechanism of IRF3 in sepsis-associated acute kidney injury via the Hippo pathway. Xingui Dai WB Human HK-2 cell

A novel drug combination of Tofacitinib and Iguratimod alleviates rheumatoid arthritis and secondary osteoporosis. INTERNATIONAL IMMUNOPHARMACOLOGY Qiang Shu IHC Rat 1:100 Synovial tissue,bone tissue

Cathepsin B aggravates atherosclerosis in ApoE-deficient mice by modulating vascular smooth muscle cell pyroptosis through NF-κB / NLRP3 signaling pathway PLoS One Hui Li IHC,WB Mouse 1:200 aorta tissue vascular smooth muscle cells (VSMCs)

TREM2 Alleviates Subarachnoid Hemorrhage-Induced Brain Injury through Attenuating Neuroinflammation and Programmed Cell Death in Vivo and in Vitro Frontiers in Bioscience-Landmark Jiaqiang Liu WB Mouse 1:1000 Temporal cortical tissue HT22 cell

• June 19-2018
• WESTERN IMMUNOBLOTTING PROTOCOL

• June 19-2018
• IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL

• June 19-2018
• IMMUNOFLUORESCENCE PROTOCOL

• September 08-2020
• FLOW-CYTOMEYRT-PROTOCOL

• May 20-2022
• Cell-Based ELISA│解您多样本WB检测之困扰

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN

• July 13-2018
• CELL-BASED-COLORIMETRIC-ELISA-PROTOCOL-FOR-TOTAL-PROTEIN

• July 13-2018
• Antibody-FAQs