Rabphilin-3A (phospho Ser237) Polyclonal Antibody
- Catalog No.：YP0891
- Applications：WB;IHC;IF;ELISA
- Reactivity：Human;Mouse;Rat
- Target：
- Rabphilin 3A
- Gene Name：
- RPH3A
- Protein Name：
- Rabphilin-3A
- Human Gene Id：
- 22895
- Human Swiss Prot No：
- Q9Y2J0
- Mouse Gene Id：
- 19894
- Mouse Swiss Prot No：
- P47708
- Rat Gene Id：
- 171039
- Rat Swiss Prot No：
- P47709
- Immunogen：
- The antiserum was produced against synthesized peptide derived from human Rabphilin 3A around the phosphorylation site of Ser237. AA range:203-252
- Specificity：
- Phospho-Rabphilin-3A (S237) Polyclonal Antibody detects endogenous levels of Rabphilin-3A protein only when phosphorylated at S237.
- Formulation：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source：
- Polyclonal, Rabbit,IgG
- Dilution：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
- Purification：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration：
- 1 mg/ml
- Storage Stability：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name：
- RPH3A;KIAA0985;Rabphilin-3A;Exophilin-1
- Observed Band(KD)：
- 75kD
- Background：
- Exocytosis of neurotransmitters and hormones is fundamental to synaptic neurotransmission and cell-cell communication. RAB3A (MIM 179390) is a small G protein that is thought to act at late stages of exocytosis, and RPH3A is a RAB3A effector (Lin et al., 2007 [PubMed 17149709]).[supplied by OMIM, Jul 2008],
- Function：
- function:Protein transport. Probably involved with Ras-related protein Rab-3A in synaptic vesicle traffic and/or synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal.,similarity:Contains 1 FYVE-type zinc finger.,similarity:Contains 1 RabBD (Rab-binding) domain.,similarity:Contains 2 C2 domains.,
- Subcellular Location：
- Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane . Cell projection, dendritic spine . Cell junction, synapse, postsynaptic cell membrane . Membrane ; Peripheral membrane protein .
- Expression：
- Brain,Lung,
- June 19-2018
- WESTERN IMMUNOBLOTTING PROTOCOL
- June 19-2018
- IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL
- June 19-2018
- IMMUNOFLUORESCENCE PROTOCOL
- September 08-2020
- FLOW-CYTOMEYRT-PROTOCOL
- May 20-2022
- Cell-Based ELISA│解您多样本WB检测之困扰
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN
- July 13-2018
- Antibody-FAQs
- Products Images
- Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Rabphilin 3A (Phospho-Ser237) Antibody
- Immunofluorescence analysis of HeLa cells, using Rabphilin 3A (Phospho-Ser237) Antibody. The picture on the right is blocked with the phospho peptide.
- Immunohistochemistry analysis of paraffin-embedded human brain, using Rabphilin 3A (Phospho-Ser237) Antibody. The picture on the right is blocked with the phospho peptide.
- Western blot analysis of lysates from RAW264.7 cells treated with Calyculin 100nM 15', using Rabphilin 3A (Phospho-Ser237) Antibody. The lane on the right is blocked with the phospho peptide.
