Stat6 (phospho Tyr641) Polyclonal Antibody
- Catalog No.：YP0256
- Applications：WB,IHC-p,IF(paraffin section),ELISA
- Reactivity：Human,Mouse,Rat
- Gene Name：
- STAT6
- Protein Name：
- Signal transducer and activator of transcription 6
- Human Gene Id：
- 6778
- Human Swiss Prot No：
- P42226
- Mouse Gene Id：
- 20852
- Mouse Swiss Prot No：
- P52633
- Immunogen：
- The antiserum was produced against synthesized peptide derived from human STAT6 around the phosphorylation site of Tyr641. AA range:608-657
- Specificity：
- Phospho-Stat6 (Y641) Polyclonal Antibody detects endogenous levels of Stat6 protein only when phosphorylated at Y641.
- Formulation：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source：
- Rabbit
- Dilution：
- Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
- Purification：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration：
- 1 mg/ml
- Storage Stability：
- -20°C/1 year
- Other Name：
- STAT6; Signal transducer and activator of transcription 6; IL-4 Stat
- MolecularWeight(Da)：
- 94135
- Observed Band(KD)：
- 94
- Background：
- signal transducer and activator of transcription 6(STAT6) Homo sapiens The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010],
- Function：
- function:Carries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling.,PTM:Tyrosine phosphorylated following stimulation by IL-4 and IL-3.,similarity:Belongs to the transcription factor STAT family.,similarity:Contains 1 SH2 domain.,subcellular location:Translocated into the nucleus in response to phosphorylation.,subunit:Forms a homodimer or a heterodimer with a related family member (By similarity). Interacts with NCOA1 via its C-terminal LXXLL motif.,
- Subcellular Location：
- nuclear chromatin,nucleus,nucleoplasm,cytoplasm,cytosol,membrane raft,
- Expression：
- Uterus,
- June 19-2018
- WESTERN IMMUNOBLOTTING PROTOCOL
- June 19-2018
- IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL
- June 19-2018
- IMMUNOFLUORESCENCE PROTOCOL
- September 08-2020
- FLOW-CYTOMEYRT-PROTOCOL
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN
- July 13-2018
- Antibody-FAQs
- Products Images
- Western blot analysis of Stat6 (phospho Tyr641) Polyclonal Antibody, using Hela,Jurkat cell treated or untreated with IFN 2500U/ml 30', 4° over night, secondary antibody(cat： RS0002 was diluted at 1:10000, 37° 1hour.
- Western Blot analysis of various cells using Phospho-Stat6 (Y641) Polyclonal Antibody
- Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using STAT6 (Phospho-Tyr641) Antibody
- Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using STAT6 (Phospho-Tyr641) Antibody. The picture on the right is blocked with the phospho peptide.
- Western blot analysis of lysates from HeLa cells treated with IL-4, using STAT6 (Phospho-Tyr641) Antibody. The lane on the right is blocked with the phospho peptide.
