Total ERK8 Cell-Based Colorimetric ELISA Kit_ImmunoWay Biotechnology Company |抗体|病理抗体|诊断抗体

Total ERK8 Cell-Based Colorimetric ELISA Kit

Other Name：
Mitogen-activated protein kinase 15 (MAP kinase 15) (MAPK 15) (EC 2.7.11.24) (Extracellular signal-regulated kinase 7) (ERK-7) (Extracellular signal-regulated kinase 8) (ERK-8)

Background：
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:The N-terminal region (1-20) is the minimal region necessary for ubiquitination and further proteosomal degradation.,domain:The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.,enzyme regulation:Activated by threonine and tyrosine phosphorylation. Inhibited by dual specificity phosphatases, such as DUSP1.,function:In vitro, phosphorylates MBP.,PTM:Dually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme. Autophosphorylated on threonine and tyrosine residues in vitro.,PTM:Ubiquitinated. Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with CSK/c-Src, ABL1, RET and TGFB1I1.,tissue specificity:Widely expressed with a maximal expression in lung and kidney.,

Function：
regulation of protein amino acid phosphorylation, positive regulation of protein amino acid phosphorylation, regulation of DNA replication, protein amino acid phosphorylation, phosphorus metabolic process, phosphate metabolic process,negative regulation of DNA replication, response to endogenous stimulus, response to hormone stimulus, negative regulation of biosynthetic process, regulation of catabolic process, positive regulation of catabolic process, response to organic substance, negative regulation of macromolecule biosynthetic process, positive regulation of phosphorus metabolic process, positive regulation of macromolecule metabolic process, negative regulation of macromolecule metabolic process, phosphorylation, regulation of phosphate metabolic process, negative regulation of cellular biosynthetic process, regulation of protein ubiquitination, positive regulation of protein ubiqu

Subcellular Location：
Cytoplasm, cytoskeleton, cilium basal body . Cell junction, tight junction . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole . Cytoplasmic vesicle, autophagosome . Golgi apparatus . Nucleus . Cytoplasm . Cytoplasm, cytoskeleton, spindle . Co-localizes to the cytoplasm only in presence of ESRRA (PubMed:21190936). Translocates to the nucleus upon activation (PubMed:20638370). At prometaphase I, metaphase I (MI), anaphase I, telophase I, and metaphase II (MII) stages, is stably detected at the spindle (By similarity). .