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免疫组化试剂
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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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Rak Polyclonal Antibody
- 货号:YT3996
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 蛋白名称:
- Tyrosine-protein kinase FRK
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human FRK. AA range:71-120
- 特异性:
- Rak Polyclonal Antibody detects endogenous levels of Rak protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- FRK;PTK5;RAK;Tyrosine-protein kinase FRK;FYN-related kinase;Nuclear tyrosine protein kinase RAK;Protein-tyrosine kinase 5
- 背景:
- The protein encoded by this gene belongs to the TYR family of protein kinases. This tyrosine kinase is a nuclear protein and may function during G1 and S phase of the cell cycle and suppress growth. [provided by RefSeq, Jul 2008],
- 功能:
- catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH2 domain.,similarity:Contains 1 SH3 domain.,tissue specificity:Restricted to cells lines derived from tissues of lymphoid, brain, breast, colon and bladder origin.,
- 细胞定位:
- Cytoplasm . Nucleus . Predominantly found in the nucleus, with a small fraction found in the cell periphery.
- 组织表达:
- Predominantly expressed in epithelial derived cell lines and tissues, especially normal liver, kidney, breast and colon.
- Western Blot analysis of various cells using Rak Polyclonal Antibody
- Immunofluorescence analysis of HUVEC cells, using FRK Antibody. The picture on the right is blocked with the synthesized peptide.
- Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using FRK Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from Jurkat cells, using FRK Antibody. The lane on the right is blocked with the synthesized peptide.