• Target：
• PI3 Kinase P85α

• Fields：
• >>EGFR tyrosine kinase inhibitor resistance;>>Endocrine resistance;>>Platinum drug resistance;>>ErbB signaling pathway;>>Ras signaling pathway;>>Rap1 signaling pathway;>>cAMP signaling pathway;>>Chemokine signaling pathway;>>HIF-1 signaling pathway;>>FoxO signaling pathway;>>Phosphatidylinositol signaling system;>>Sphingolipid signaling pathway;>>Phospholipase D signaling pathway;>>Autophagy - animal;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>AMPK signaling pathway;>>Apoptosis;>>Longevity regulating pathway;>>Longevity regulating pathway - multiple species;>>Cellular senescence;>>Axon guidance;>>VEGF signaling pathway;>>Osteoclast differentiation;>>Focal adhesion;>>Signaling pathways regulating pluripotency of stem cells;>>Platelet activation;>>Neutrophil extracellular trap formation;>>Toll-like receptor signaling pathway;>>C-type lectin receptor signaling pathway;>>JAK-STAT signaling pathway;>>Natural killer cell mediated cytotoxicity;>>T cell receptor signaling pathway;>

• Gene Name：
• PIK3R1

• Protein Name：
• Phosphatidylinositol 3-kinase regulatory subunit alpha

• Human Gene Id：
• 5295

• Human Swiss Prot No：
• P27986

• Mouse Gene Id：
• 18708

• Mouse Swiss Prot No：
• P26450

• Rat Gene Id：
• 25513

• Rat Swiss Prot No：
• Q63787

• Immunogen：
• Synthesized peptide derived from PI 3-kinase p85α . at AA range: 550-630

• Specificity：
• This antibody detects endogenous levels of PI 3-kinase p85α.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• PIK3R1;GRB1;Phosphatidylinositol 3-kinase regulatory subunit alpha;PI3-kinase regulatory subunit alpha;PI3K regulatory subunit alpha;PtdIns-3-kinase regulatory subunit alpha;Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha;PI3-kinase subunit p85-alpha;PtdIns-3-kinase regulatory subunit p85-alpha

• Observed Band(KD)：
• 54kD,83kD

• Background：
• Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011],

• Function：
• disease:Defects in PIK3R1 are a cause of severe insulin resistance.,domain:The SH3 domain mediates the binding to CBLB, and to HIV-1 Nef.,function:Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.,PTM:Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.,similarity:Belongs to the PI3K p85 subunit family.,similarity:Contains 1 Rho-GAP domain.,similarity:Contains 1 SH3 domain.,similarity:Contains 2 SH2 domains.,subunit:Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunits. Interacts with phosphorylated TOM1L1. Interacts with phosphorylat

• Subcellular Location：
• nucleus,cytoplasm,cis-Golgi network,cytosol,plasma membrane,cell-cell junction,phosphatidylinositol 3-kinase complex,phosphatidylinositol 3-kinase complex, class IA,membrane,perinuclear endoplasmic reticulum membrane,

• Expression：
• Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).

Proteomic Study on the Reproductive Toxicity of Tripterygium Glycosides in Rats WB Rat testis tissue /

Melatonin treatment improves human umbilical cord mesenchymal stem cell therapy in a mouse model of type II diabetes mellitus via the PI3K/AKT signaling pathway. Stem Cell Research & Therapy2022 Dec;13(1):1-15. Human,Mouse 1:1200 liver tissue hUC-MSC

Irisin Suppresses Nicotine-Mediated Atherosclerosis by Attenuating Endothelial Cell Migration, Proliferation, Cell Cycle Arrest, and Cell Senescence. Frontiers in Cardiovascular Medicine2022; 9: 851603. Human 1:4000 HUVECs

Effects of D-Chiro-Inositol on Glucose Metabolism in db/db Mice and the Associated Underlying Mechanisms. Frontiers in Pharmacology Front Pharmacol. 2020 Mar;11:354 IHC Mouse 1:200 liver tissues

Induction of PI3K/Akt-Mediated Apoptosis in Osteoclasts Is a Key Approach for Buxue Tongluo Pills to Treat Osteonecrosis of the Femoral Head.. Frontiers in Pharmacology Front Pharmacol. 2021 Nov;12:729909-729909 WB Mouse 1:500，1:1000 RAW 264.7 cell

iTRAQ-Based Proteomics Reveals Gu-Ben-Fang-Xiao Decoction Alleviates Airway Remodeling via Reducing Extracellular Matrix Deposition in a Murine Model of Chronic Remission Asthma. Frontiers in Pharmacology Front Pharmacol. 2021; 12: 588588 WB Mouse 1:1000 Lung

5‐FU inhibits migration and invasion of CRC cells through PI3K/AKT pathway regulated by MARCH1. CELL BIOLOGY INTERNATIONAL Cell Biol Int. 2021 Feb;45(2):368-381 WB Human Colorectal cancer(CRC ) tissues sw480s,DLD-1s,NCM460s

An Integrated Approach Based on Network Pharmacology Combined with Experimental Verification Reveals AMPK/PI3K/Akt Signaling is an Important Way for the Anti-Type 2 Diabetic Activity of Silkworm Excrement. Diabetes Metabolic Syndrome and Obesity-Targets and Therapy Diabet Metab Synd Ob. 2021 Feb;14:601-616 WB Human 1:1000 HepG2 cell

Scutellarin Prevents Nonalcoholic Fatty Liver Disease (NAFLD) and Hyperlipidemia via PI3K/AKT-Dependent Activation of Nuclear Factor (Erythroid-Derived 2)-Like 2 (Nrf2) in Rats. MEDICAL SCIENCE MONITOR Med Sci Monitor. 2017; 23: 5599–5612 WB Rat 1: 400 liver tissue

Effects of Agriophyllum squarrosum extracts on glucose metabolism in KKAy mice and the associated underlying mechanisms. JOURNAL OF ETHNOPHARMACOLOGY J Ethnopharmacol. 2019 Sep;241:112009 WB Mouse 1:1000 liver

Long Non-coding RNA GAS5 Maintains Insulin Secretion by Regulating Multiple miRNAs in INS-1 832/13 Cells. Frontiers in Molecular Biosciences Front Mol Biosci. 2020; 7: 559267 WB Rat 1 : 1000 INS-1 832/13 cell

Irisin ameliorates nicotine-mediated atherosclerosis via inhibition of the PI3K pathway. Annals of Translational Medicine Ann Transl Med. 2021 May; 9(9): 805 WB Mouse 1:10000 Aortic

The role and regulatory mechanism of autophagy in hippocampal nerve cells of piglet damaged by deoxynivalenol. TOXICOLOGY IN VITRO Toxicol In Vitro. 2020 Aug;66:104837 WB Pig 1:1000 piglet hippocampal nerve cells (PHNCs)

Sattar, Hamid, et al. "Selenoprotein-U (SelU) knockdown triggers autophagy through PI3K–Akt–mTOR pathway inhibition in rooster Sertoli cells." Metallomics 10.7 (2018): 929-940.

Yang, Jie, et al. "Selenium deficiency-induced thioredoxin suppression and thioredoxin knock down disbalanced insulin responsiveness in chicken cardiomyocytes through PI3K/Akt pathway inhibition." Cellular signalling 38 (2017): 192-200.

Wang, Wei-wei, et al. "Telmisartan reduces atrial arrhythmia susceptibility through the regulation of RAS–ERK and PI3K–Akt–eNOS pathways in spontaneously hypertensive rats." Canadian journal of physiology and pharmacology 93.8 (2015): 657-665.

Irisin ameliorates nicotine-mediated atherosclerosis via inhibition of the PI3K pathway. Annals of Translational Medicine Ann Transl Med. 2021 May; 9(9): 805 WB Mouse 1:10000 Aortic

5‐FU inhibits migration and invasion of CRC cells through PI3K/AKT pathway regulated by MARCH1. CELL BIOLOGY INTERNATIONAL Cell Biol Int. 2021 Feb;45(2):368-381 WB Human Colorectal cancer(CRC ) tissues sw480s,DLD-1s,NCM460s

5‐FU inhibits migration and invasion of CRC cells through PI3K/AKT pathway regulated by MARCH1. CELL BIOLOGY INTERNATIONAL Cell Biol Int. 2021 Feb;45(2):368-381 WB Human Colorectal cancer(CRC ) tissues sw480s,DLD-1s,NCM460s

Irisin ameliorates nicotine-mediated atherosclerosis via inhibition of the PI3K pathway. Annals of Translational Medicine Ann Transl Med. 2021 May; 9(9): 805 WB Mouse 1:10000 Aortic

Arsenic Trioxide-loaded nanoparticles Enhance the Chemosensitivity of Gemcitabine in Pancreatic Cancer via Reversal of Pancreatic Stellate Cells Desmoplasia through Targeting AP4/Galectin-1 Pathway Biomaterials Science Kaihong Huang WB Human

Amelioration of Lung Fibrosis by Total Flavonoids of Astragalus via Inflammatory Modulation and Epithelium Regeneration AMERICAN JOURNAL OF CHINESE MEDICINE Min Li WB Human,Mouse lung tissues A549 cells

Crosstalk between autophagy inhibitor and salidroside-induced apoptosis: A novel strategy for autophagy-based treatment of hepatocellular cancer. INTERNATIONAL IMMUNOPHARMACOLOGY Haixiang Su WB Human 1:1000 HepG2 cell,97H cell

Multi-omics revealed the mechanisms of Codonopsis pilosula aqueous extract in improving UC through blocking abnormal activation of PI3K/Akt signaling pathway. JOURNAL OF ETHNOPHARMACOLOGY Fude Yang IHC Rat intestinal mucosa

Cucurbitacin E reduces IL-1β-induced inflammation and cartilage degeneration by inhibiting the PI3K/Akt pathway in osteoarthritic chondrocytes Journal of Translational Medicine Wang Lin WB Human 1:1000 Chondrocytes

MiR-21-5p ameliorates Busulfan-induced testicular dysfunction and maintains spermatogenesis1 Journal of Integrative Agriculture Meng-fei ZHANG WB Goat Male germ stem cells (mGSCs)

Synthesis of peiminine derivatives and its effect on A549 lung cancer-bearing mice Chemical Biology & Drug Design Xin Jin WB Mouse A549 cell-Xenograft

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