• Target：
• Actin, smooth muscle (SMA)

• Fields：
• >>Vascular smooth muscle contraction;>>Apelin signaling pathway;>>Relaxin signaling pathway

• Gene Name：
• ACTA2

• Protein Name：
• Actin aortic smooth muscle

• Human Gene Id：
• 59

• Human Swiss Prot No：
• P62736

• Mouse Gene Id：
• 11475

• Mouse Swiss Prot No：
• P62737

• Rat Gene Id：
• 81633

• Rat Swiss Prot No：
• P62738

• Immunogen：
• Synthesized peptide derived from the N-terminal region of human α-SMA. AA range: 84-134

• Specificity：
• α-SMA Polyclonal Antibody detects endogenous levels of α-SMA protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• ACTA2;ACTSA;ACTVS;GIG46;Actin; aortic smooth muscle;Alpha-actin-2;Cell growth-inhibiting gene 46 protein

• Observed Band(KD)：
• 42kD

• Background：
• The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008],

• Function：
• disease:Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.,function:Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.,miscellaneous:In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actin

• Subcellular Location：
• Cytoplasm, cytoskeleton.

• Expression：
• Pituitary,Uterus,

Knockdown of SOX9 alleviates tracheal fibrosis through the Wnt/β-catenin signaling pathway

HMGB1 contributes to allergen-induced airway remodeling in a murine model of chronic asthma by modulating airway inflammation and activating lung fibroblasts. Cellular & Molecular Immunology Cell Mol Immunol. 2014 Aug;12(4):409-423 IHC Mouse 1∶300 left lung

Ginsenoside Rg2 alleviates myocardial fibrosis by regulating TGF-β1/Smad signalling pathway. PHARMACEUTICAL BIOLOGY Pharm Biol. 2021;59(1):106-113 WB Rat 1:2000 Myocardial

Resveratrol inhibits monocrotaline-induced pulmonary arterial remodeling by suppression of SphK1-mediated NF-κB activation. LIFE SCIENCES Life Sci. 2018 Oct;210:140 WB Rat 1:200 lung PASMCs

PDGF-mediated PI3K/AKT/β-catenin signaling regulates gap junctions in corpus cavernosum smooth muscle cells. EXPERIMENTAL CELL RESEARCH Exp Cell Res. 2018 Jan;362:252 IF Rat 1:100 SMCs

HDAC3 inhibitor suppresses endothelial-to-mesenchymal transition via modulating inflammatory response in atherosclerosis. BIOCHEMICAL PHARMACOLOGY Biochem Pharmacol. 2021 Oct;192:114716 WB Human，Mouse Aortas HUVEC cell

NOGO-B promotes EMT in lung fibrosis via MMP14 mediates free TGF-beta1 formation. Oncotarget Oncotarget. 2017 Sep 19; 8(41): 71024–71037 WB Human 1:1000 A549 cell

SIRT6 inhibits endothelial-to-mesenchymal transition through attenuating the vascular endothelial inflammatory response. INTERNATIONAL IMMUNOPHARMACOLOGY Int Immunopharmacol. 2021 Dec;101:108240 WB Human HUVECs

The mechanism of mulberry leaves against renal tubular interstitial fibrosis through ERK1/2 signaling pathway was predicted by network pharmacology and validated in human tubular epithelial cells. PHYTOTHERAPY RESEARCH 2019 Jun 17 WB Human 1:1000 HK-2 cell

Elevated c-fos expression is correlated with phenotypic switching of human vascular smooth muscle cells derived from lower limb venous varicosities. Journal of Vascular Surgery-Venous and Lymphatic Disorders J Vasc Surg-Venous L. 2021 Jan;9:242 WB Human 1:10000 VSMCs

Huiyang Shengji Extract Improve Chronic Nonhealing Cutaneous through the TGF-β1/Smad3 Signaling Pathway. Evidence-based Complementary and Alternative Medicine Evid-Based Compl Alt. 2021;2021:8881565 WB Human Human skin fibroblasts cells (HSFs)

Lipid accumulation is ahead of epithelial-to-mesenchymal transition and therapeutic intervention by acetyl-CoA carboxylase 2 silence in diabetic nephropathy. METABOLISM-CLINICAL AND EXPERIMENTAL 2014 Feb 27 WB Human HK-2 cell

Potential role of MG53 in the regulation of transforming-growth-factor-β1-induced atrial fibrosis and vulnerability to atrial fibrillation. EXPERIMENTAL CELL RESEARCH Exp Cell Res. 2018 Jan;362:436 WB Human 1:1000 right atrial appendages atrial fibroblasts

Salvianolic Acid B-Alleviated Angiotensin II Induces Cardiac Fibrosis by Suppressing NF-κB Pathway In Vitro. MEDICAL SCIENCE MONITOR Med Sci Monitor. 2018; 24: 7654–7664 WB Rat 1: 1000 cardiac fibroblasts (CFBs)

Assessing activation of hepatic stellate cells by 99mTc-3PRGD2 scintigraphy targeting integrin αvβ3: a feasibility study. NUCLEAR MEDICINE AND BIOLOGY 2014 Nov 26 WB Rat liver

miR‑221 targets HMGA2 to inhibit bleomycin‑induced pulmonary fibrosis by regulating TGF‑β1/Smad3-induced EMT. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 2016 Aug 11 WB Human A549 cell,HBE cell

Epigenetic repression of Krüppel-like factor 4 through Dnmt1 contributes to EMT in renal fibrosis. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 2015 Apr 20 WB Human HK-2 cell

Kangfuxin Oral Liquid Attenuates Bleomycin-Induced Pulmonary Fibrosis via the TGF-β1/Smad Pathway. Evidence-based Complementary and Alternative Medicine Evid-Based Compl Alt. 2019;2019:5124026 IHC Rat 1 : 200 Lung tissue

miR-19 targets PTEN and mediates high mobility group protein B1(HMGB1)-induced proliferation and migration of human airway smooth muscle cells. PLoS One 2019 Jun 27 WB Human HASM cell

Immortalized bovine mammary epithelial cells express stem cell markers and differentiate in vitro. CELL BIOLOGY INTERNATIONAL 2016 Jun 19 ICC Human MCF-7 cell

Longitudinal Immune Profiling Highlights CD4+ T Cell Exhaustion Correlated with Liver Fibrosis in Schistosoma japonicum Infection JOURNAL OF IMMUNOLOGY Zhiqiang Qin IF Mouse livers

Regulation of endothelial-to-mesenchymal transition by histone deacetylase 3 posttranslational modifications in neointimal hyperplasia Annals of Translational Medicine Weirong Wang WB Human human umbilical vein endothelial cells (HUVECs)

Cryptotanshinone alleviates lipopolysaccharide and cigarette smoke-induced chronic obstructive pulmonary disease in mice via the Keap1/Nrf2 axis. Jianwen Chen WB，IHC Mouse lung

Inhibición de la Proliferación, Migración e Invasión de Fibroblastos Queloides por miR-183-5p Mediante la Regulación Negativa de EGR1 INTERNATIONAL JOURNAL OF MORPHOLOGY Mingxi Li WB Human 1:1000 Keloid fibroblasts (KFs)

Nobiletin Alleviated Epithelial–Mesenchymal Transition of Hepatocytes in Liver Fibrosis Based on Autophagy—Hippo/YAP Pathway MOLECULAR NUTRITION & FOOD RESEARCH Yuting Hao WB Mouse,Human liver tissue L02 cell

Gut Microbiota Plays Essential Roles in Soyasaponin's Preventive Bioactivities against Steatohepatitis in the Methionine and Choline Deficient (MCD) Diet-Induced Non-Alcoholic Steatohepatitis (NASH) Mice MOLECULAR NUTRITION & FOOD RESEARCH Chuhong Su WB Mouse liver tissue

2-Dodecyl-6-Methoxycyclohexa-2, 5-Diene-1, 4-Dione isolated from Averrhoa carambola L. root inhibits high glucose-induced EMT in HK-2 cells through targeting the regulation of miR-21–5p/Smad7 signaling pathway BIOMEDICINE & PHARMACOTHERAPY Jingyi Li WB Human 1:1000 HK-2 cell

• June 19-2018
• WESTERN IMMUNOBLOTTING PROTOCOL

• June 19-2018
• IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL

• June 19-2018
• IMMUNOFLUORESCENCE PROTOCOL

• September 08-2020
• FLOW-CYTOMEYRT-PROTOCOL

• May 20-2022
• Cell-Based ELISA│解您多样本WB检测之困扰

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN

• July 13-2018
• CELL-BASED-COLORIMETRIC-ELISA-PROTOCOL-FOR-TOTAL-PROTEIN

• July 13-2018
• Antibody-FAQs