Smad1/5/9 Polyclonal Antibody_ImmunoWay Biotechnology Company |抗体|病理抗体|诊断抗体

Smad1/5/9 Polyclonal Antibody

Catalog No.：YT4325
Applications：WB;IHC;IF;ELISA
Reactivity：Human;Mouse;Rat

Description
References ( 185 )
Protocol

Target：
Smad1/5/9

Fields：
>>TGF-beta signaling pathway;>>Hippo signaling pathway;>>Signaling pathways regulating pluripotency of stem cells;>>Transcriptional misregulation in cancer

Gene Name：
SMAD1/SMAD5/SMAD9

Protein Name：
Mothers against decapentaplegic homolog 1/Mothers against decapentaplegic homolog 5/Mothers against decapentaplegic homolog 9

Human Gene Id：
4086/4090/4093

Human Swiss Prot No：
Q15797/Q99717/O15198

Mouse Gene Id：
17125/17129/55994

Rat Gene Id：
59328/85435

Rat Swiss Prot No：
P97588/Q9R1V3/O54835

Immunogen：
The antiserum was produced against synthesized peptide derived from human Smad1/5/9. AA range:10-59

Specificity：
Smad1/5/9 Polyclonal Antibody detects endogenous levels of Smad1/5/9 protein.

Formulation：
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：
Polyclonal, Rabbit,IgG

Dilution：
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

Purification：
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：
1 mg/ml

Storage Stability：
-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：
SMAD1;BSP1;MADH1;MADR1;Mothers against decapentaplegic homolog 1;MAD homolog 1;Mothers against DPP homolog 1;JV4-1;Mad-related protein 1;SMAD family member 1;SMAD 1;Smad1;hSMAD1;Transforming growth factor-beta-signaling protein

Observed Band(KD)：
56kD

Background：
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-med

Function：
function:Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD).,PTM:Phosphorylated on serine by BMP type 1 receptor kinase.,PTM:Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.,similarity:Belongs to the dwarfin/SMAD family.,similarity:Contains 1 MH1 (MAD homology 1) domain.,similarity:Contains 1 MH2 (MAD homology 2) domain.,subcellular location:Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.,subunit:Interacts with HGS, NANOG and ZCCHC12 (By similarity). May form trimers with another SMAD1 and the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit, CREB-binding protein (CBP), p300, SMURF1, SMURF2 and HOXC8. Associates with ZNF423 or ZNF521 in response to BMP2 leading to activate transcription of BMP target genes. Interacts with LBXCOR1.,

Subcellular Location：
Cytoplasm . Nucleus . Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4 (PubMed:15647271). Co-localizes with LEMD3 at the nucleus inner membrane (PubMed:15647271). Exported from the nucleus to the cytoplasm when dephosphorylated (By similarity). .

Expression：
Ubiquitous. Highest expression seen in the heart and skeletal muscle.

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Exploration of potential mechanism of interleukin-33 up-regulation caused by 1,4-naphthoquinone black carbon in RAW264.7 cells. SCIENCE OF THE TOTAL ENVIRONMENT2022 Aug;835:155357. Mouse RAW264.7 cell

G-CSF attenuates neuroinflammation and neuronal apoptosis via the mTOR/p70SK6 signaling pathway in neonatal Hypoxia-Ischemia rat model. BRAIN RESEARCH 2020 Apr 01 WB Rat 1:1000 brain

HMGB1 binding to receptor for advanced glycation end products enhances inflammatory responses of human bronchial epithelial cells by activating p38 MAPK and ERK1/2. MOLECULAR AND CELLULAR BIOCHEMISTRY Mol Cell Biochem. 2015 Jul;405(1):63-71 WB Human HBECs

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Hippocampal miR-206-3p participates in the pathogenesis of depression via regulating the expression of BDNF. PHARMACOLOGICAL RESEARCH Pharmacol Res. 2021 Dec;174:105932 WB Mouse 1:2000 Hippocampus tissue

Tetraethylthiuram disulphide alleviates pulmonary fibrosis through modulating transforming growth factor-β signalling. PHARMACOLOGICAL RESEARCH Pharmacol Res. 2021 Dec;174:105923 WB Mouse Lung HEK293T cells

Licoflavone A Suppresses Gastric Cancer Growth and Metastasis by Blocking the VEGFR-2 Signaling Pathway. Journal of Oncology2022;2022:5497991. Human 1 : 1000 MKN-45 cell

Exosomes-transmitted miR-7 reverses gefitinib resistance by targeting YAP in non-small-cell lung cancer. PHARMACOLOGICAL RESEARCH Pharmacol Res. 2021 Mar;165:105442 WB Human，Mouse PC9 cell-Xenograft H1975 cell，PC9 cell

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Selenium Yeast Alleviates Ochratoxin A-Induced Apoptosis and Oxidative Stress via Modulation of the PI3K/AKT and Nrf2/Keap1 Signaling Pathways in the Kidneys of Chickens. Oxidative Medicine and Cellular Longevity Oxid Med Cell Longev. 2020;2020:4048706 WB Chicken kidney

HnRNP-L mediates bladder cancer progression by inhibiting apoptotic signaling and enhancing MAPK signaling pathways. Oncotarget Oncotarget. 2017 Feb 21; 8(8): 13586–13599 WB Human UM-UC-3 cell,EJ cell

3-Epipachysamine B suppresses proliferation and induces apoptosis of breast cancer cell via PI3K/AKT/mTOR signaling pathway. LIFE SCIENCES Life Sci. 2021 Nov;285:119995 WB Human 1:800 MCF-7 cell,MDA-MB-231 cell

Capecitabine Can Induce T Cell Apoptosis: A Potential Immunosuppressive Agent With Anti-Cancer Effect. Frontiers in Immunology Front Immunol. 2021; 12: 737849 WB Mouse Bone marrow cells

Negative Regulation of RNF90 on RNA Virus-Triggered Antiviral Immune Responses Targeting MAVS. Frontiers in Immunology Front Immunol. 2021; 12: 730483 WB Mouse,Human BMDMs,HaCaT Cell

The Chlamydia psittaci Inclusion Membrane Protein 0556 Inhibits Human Neutrophils Apoptosis Through PI3K/AKT and NF-κB Signaling Pathways. Frontiers in Immunology Front Immunol. 2021; 12: 694573 IF Human 1:50 Human polymorphonuclear neutrophils (hPMN)

Relationship between PI3K/mTOR/RhoA pathway-regulated cytoskeletal rearrangements and phagocytic capacity of macrophages. BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH Braz J Med Biol Res. 2020 Jun;53: WB Mouse 1:500 RAW264.7 cells

Allogeneic adipose-derived stem cell transplantation on knee osteoarthritis rats and its effect on MMP-13 and DDR2. Experimental and Therapeutic Medicine 2019 May 08 IHC Rat knee joint tissue

miR-378 inhibits cell growth and enhances apoptosis in human myelodysplastic syndromes. INTERNATIONAL JOURNAL OF ONCOLOGY 2016 Nov 01 WB Human SKM-1 cell

Therapeutic Effects of (5R)-5-Hydroxytriptolide on Fibroblast-Like Synoviocytes in Rheumatoid Arthritis via lncRNA WAKMAR2/miR-4478/E2F1/p53 Axis. Frontiers in Immunology Front Immunol. 2021 Feb;12:296 ELISA Human Peripheral blood mononuclear cells (PBMCs)

Zdhhc2 Is Essential for Plasmacytoid Dendritic Cells Mediated Inflammatory Response in Psoriasis. Frontiers in Immunology Front Immunol. 2021 Jan;0:3442 WB Human CAL-1 WT cell, CAL-1 zDHHC2−/− cell

SRGAP1, a crucial target of miR-340 and miR-124, functions as a potential oncogene in gastric tumorigenesis. ONCOGENE 2017 Dec 13 WB,IF Human SGC-7901 cell, MKN28 cell, MGC-803 cell

Nucleus accumbens-associated protein-1 promotes glycolysis and survival of hypoxic tumor cells via the HDAC4-HIF-1α axis. ONCOGENE 2017 Mar 20 WB Human HeLa cell, SKOV3 cell

Methyl-CpG-binding protein 2 drives the Furin/TGF-β1/Smad axis to promote epithelial–mesenchymal transition in pancreatic cancer cells. Oncogenesis Oncogenesis. 2020 Aug;9(8):1-14 WB Human 293T cell , PANC1 cell , PaTu8988 cell,SW1990 cell

Endoplasmic reticulum stress-mediated autophagy activation is involved in cadmium-induced ferroptosis of renal tubular epithelial cells. FREE RADICAL BIOLOGY AND MEDICINE Free Radical Bio Med. 2021 Nov;175:236 WB Mouse Renal tissues TCMK-1 cells

Glycyrrhetinic acid induces oxidative/nitrative stress and drives ferroptosis through activating NADPH oxidases and iNOS, and depriving glutathione in triple-negative breast cancer cells. FREE RADICAL BIOLOGY AND MEDICINE Free Radical Bio Med. 2021 Sep;173:41 WB Human 1:1000 MDA-MB-231 cells

C–C motif ligand 8 promotes atherosclerosis via NADPH oxidase 2/reactive oxygen species-induced endothelial permeability increase. FREE RADICAL BIOLOGY AND MEDICINE Free Radical Bio Med. 2021 May;167:181 WB，IF Human Human aortic endothelial cells (HAECs)

Angiogenesis is promoted by exosomal DPP4 derived from 5-fluorouracil-resistant colon cancer cells. CANCER LETTERS Cancer Lett. 2021 Jan;497:190 WB Human 1 : 500 HCT-8 cell，HUVECs

Insulin-like growth factor-1 receptor induces immunosuppression in lung cancer by upregulating B7–H4 expression through the MEK/ERK signaling pathway. CANCER LETTERS Cancer Lett. 2020 Aug;485:14 WB Human A549 cell,SPC-A-1 cell, LLC cell

DCLK1 promotes epithelial‐mesenchymal transition via the PI3K/Akt/NF‐κB pathway in colorectal cancer. INTERNATIONAL JOURNAL OF CANCER Int J Cancer. 2018 May;142(10):2068-2079 WB Human HCT116 cell ,SW480 cell

Corticosterone Inhibits LPS-Induced NLRP3 Inflammasome Priming in Macrophages by Suppressing Xanthine Oxidase. MEDIATORS OF INFLAMMATION Mediat Inflamm. 2020;2020:6959741 WB Mouse RAW264.7 cells

The oncoprotein HBXIP enhances migration of breast cancer cells through increasing filopodia formation involving MEKK2/ERK1/2/Capn4 signaling. CANCER LETTERS 2014 Oct 07 WB Human MCF-7 cell

A size switchable nanoplatform for targeting the tumor microenvironment and deep tumor penetration. Nanoscale Nanoscale. 2018 May;10(21):9935-9948 IHC Mouse 1:100 4T1 xenograft

Transmembrane Domain Targeting Peptide Antagonizing ErbB2/Neu Inhibits Breast Tumor Growth and Metastasis. Cell Reports 2014 Sep 15 ELISA Mouse NT193 Cell

Herpes Simplex Virus 1 UL36USP Antagonizes Type I Interferon-Mediated Antiviral Innate Immunity. JOURNAL OF VIROLOGY J Virol. 2018 Oct;: IP Human HEK293T cell

Upregulation of IL-15 in the placenta alters trophoblasts behavior contributing to gestational diabetes mellitus. Cell and Bioscience Cell Biosci. 2021 Dec;11(1):1-15 IF Human Placenta

Nucleotide de novo synthesis increases breast cancer stemness and metastasis via cGMP-PKG-MAPK signaling pathway. PLOS BIOLOGY Plos Biol. 2020 Nov;18(11):e3000872 WB Mouse 1:1000 4T1 cell,4TO7O cell,4TO7cell

Hedgehog signaling promotes sorafenib resistance in hepatocellular carcinoma patient-derived organoids. JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH J Exp Clin Canc Res. 2020 Dec;39(1):1-15 WB Human HCC patient-derived organoid (PDO)

Novel Mouse miRNA Chr13_novelMiR7354-5p Improves Bone-Marrow-Derived Mesenchymal Stem Cell Differentiation into Insulin-Producing Cells. Molecular Therapy-Nucleic Acids Mol Ther-Nucl Acids. 2020 Mar;19:1110 WB Mouse BM-MSCs

A Discovery of Clinically Approved Formula FBRP for Repositioning to Treat HCC by Inhibiting PI3K/AKT/NF-κB Activation. Molecular Therapy-Nucleic Acids Mol Ther-Nucl Acids. 2020 Mar;19:890 WB Rat Liver Tissues

Exosomes Serve as Nanoparticles to Deliver Anti-miR-214 to Reverse Chemoresistance to Cisplatin in Gastric Cancer. MOLECULAR THERAPY 2018 Jan 08 WB Human 1:500 SGC7901 cell,HEK293T cell

Development of a novel thymidylate synthase (TS) inhibitor capable of up-regulating P53 expression and inhibiting angiogenesis in NSCLC. Journal of Advanced Research 2020 Jul 25 WB Human A549 cell， PC-9 cells

Gastric cancer-derived exosomal miR-135b-5p impairs the function of Vγ9Vδ2 T cells by targeting specificity protein 1. CANCER IMMUNOLOGY IMMUNOTHERAPY Cancer Immunol Immun. 2022 Feb;71(2):311-325 WB Human MGC803 cell

Structural characterization of a water-soluble polysaccharide from Angelica dahurica and its antitumor activity in H22 tumor-bearing mice. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES Int J Biol Macromol. 2021 Dec;193:219 FC Mouse Peripheral blood

Adenovirus-Mediated CCR7 and BTLA Overexpression Enhances Immune Tolerance and Migration in Immature Dendritic Cells. Biomed Research International Biomed Res Int. 2017;2017:3519745 WB Mouse 1 : 1000 imDCs

Sex-determining region Y box 4 (SOX4) suppresses Hepatitis B virus replication by inhibiting hepatocyte nuclear factor 4α expression. ANTIVIRAL RESEARCH Antivir Res. 2020 Apr;176:104745 WB Human HuH7 cell

HMGA1-TRIP13 axis promotes stemness and epithelial mesenchymal transition of perihilar cholangiocarcinoma in a positive feedback loop dependent on c-Myc. JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH J Exp Clin Canc Res. 2021 Dec;40(1):1-18 WB Human pCCA tissues ，adjacent normal tissue QBC939 cell，FRH0201 cell

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Periodontal ligament‐like tissue regeneration with drilled porous decalcified dentin matrix sheet composite. ORAL DISEASES Oral Dis. 2018 Apr;24(3):429-441 IF Human HDFCs

Rab34 regulates adhesion, migration, and invasion of breast cancer cells. ONCOGENE Oncogene. 2018 Apr;37(27):3698-3714 IP Human MCF7 cell

The adipose-derived mesenchymal stem cell secretome promotes hepatic regeneration in miniature pigs after liver ischaemia-reperfusion combined with partial resection. Stem Cell Research & Therapy Stem Cell Res Ther. 2021 Dec;12(1):1-12 WB Pig Liver

Autoantibody-Mediated Erythrophagocytosis Increases Tuberculosis Susceptibility in HIV Patients. mBio Mbio. 2020 Feb;: FC Human 1:100 THP-1 macrophages

ERK/Drp1‐dependent mitochondrial fission contributes to HMGB1‐induced autophagy in pulmonary arterial hypertension. CELL PROLIFERATION Cell Proliferat. 2021 Jun;54(6):e13048 WB Rat 1 : 1000 Lung PASMCs

Baicalin Attenuates Blood-Brain Barrier Disruption and Hemorrhagic Transformation and Improves Neurological Outcome in Ischemic Stroke Rats with Delayed t-PA Treatment: Involvement of ONOO − -MMP-9 Pathway. Translational Stroke Research Transl Stroke Res. 2018 Oct;9(5):515-529 IHC Rat brain

Kaposi’s sarcoma-associated herpesvirus vFLIP promotes MEndT to generate hybrid M/E state for tumorigenesis. PLoS Pathogens Plos Pathog. 2021 Dec;17(12):e1009600 WB Human PDLSCs (2-D) ,PDLSC spheroid (3-D)

Kaposi’s sarcoma-associated herpesvirus vFLIP promotes MEndT to generate hybrid M/E state for tumorigenesis. PLoS Pathogens Plos Pathog. 2021 Dec;17(12):e1009600 IF Human Kaposi’s sarcoma (KS)

Kaposi’s sarcoma-associated herpesvirus vFLIP promotes MEndT to generate hybrid M/E state for tumorigenesis. PLoS Pathogens Plos Pathog. 2021 Dec;17(12):e1009600 IHC Human Kaposi’s sarcoma (KS)

Kaposi’s sarcoma-associated herpesvirus promotes mesenchymal-to-endothelial transition by resolving the bivalent chromatin of PROX1 gene. PLoS Pathogens Plos Pathog. 2021 Sep;17(9):e1009847 WB Mouse 1:1000 Periodontal ligament stem cells (PDLSCs)

Seselin ameliorates inflammation via targeting Jak2 to suppress the proinflammatory phenotype of macrophages. BRITISH JOURNAL OF PHARMACOLOGY Brit J Pharmacol. 2019 Jan;176(2):317-333 WB Mouse RAW 264.7 cell

Micro/nano-textured hierarchical titanium topography promotes exosome biogenesis and secretion to improve osseointegration. JOURNAL OF NANOBIOTECHNOLOGY J Nanobiotechnol. 2021 Dec;19(1):1-16 WB Human 1 : 1000 hBMSCs

Photothermal Therapeutic Response of Cancer Cells to Aptamer–Gold Nanoparticle-Hybridized Graphene Oxide under NIR Illumination. ACS Applied Materials & Interfaces 2015 Feb 27 WB Human MCF-7 cell

Increased expression of HSP27 inhibits invasion and metastasis in human esophageal squamous cell carcinoma. TUMOR BIOLOGY Tumor Biol. 2014 Jul;35(7):6999-7007 WB,IHC Human 1:100，1:300 esophageal squamous cell carcinoma (ESCC) tissue

3,3'-Diindolylmethane induces gastric cancer cells death via STIM1 mediated store-operated calcium entry. International Journal of Biological Sciences Int J Biol Sci. 2021; 17(5): 1217–1233 WB Human 1 : 1000 BGC-823 cell，SGC-7901 gastric cancer cell

Ginsenoside Rg3 Inhibits the Growth of Osteosarcoma and Attenuates Metastasis through the Wnt/β-Catenin and EMT Signaling Pathway. Evidence-based Complementary and Alternative Medicine Evid-Based Compl Alt. 2020;2020:6065124 WB Human MG63 cell，143B cell

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Overexpression of CXCL3 can enhance the oncogenic potential of prostate cancer. INTERNATIONAL UROLOGY AND NEPHROLOGY Int Urol Nephrol. 2016 May;48(5):701-709 ELISA Human 1:10000 PC-3 cell, DU145 cell, LNCaP cell, WPMY-1 cell,RWPE-1 cell

miR-378 inhibits cell growth and enhances apoptosis in human myelodysplastic syndromes. INTERNATIONAL JOURNAL OF ONCOLOGY 2016 Nov 01 WB Human SKM-1 cell

Changes in the Gut Microbiome Contribute to the Development of Behcet’s Disease via Adjuvant Effects. Frontiers in Cell and Developmental Biology Front Cell Dev Biol. 2021 Sep;0:2482 WB Mouse 1:1000 Colon

Fexofenadine Protects Against Intervertebral Disc Degeneration Through TNF Signaling. Frontiers in Cell and Developmental Biology Front Cell Dev Biol. 2021; 9: 687024 WB Human，Mouse，Rat 1:1000 Nucleus pulposus （NP）tissues

GADD45B Is a Potential Diagnostic and Therapeutic Target Gene in Chemotherapy-Resistant Prostate Cancer. Frontiers in Cell and Developmental Biology Front Cell Dev Biol. 2021; 9: 716501 IHC，WB Human 1:100，1:500 Prostate cancer (PCa) tissue

The Regulatory NOD-Like Receptor NLRC5 Promotes Ganglion Cell Death in Ischemic Retinopathy by Inducing Microglial Pyroptosis. Frontiers in Cell and Developmental Biology Front Cell Dev Biol. 2021 May;0:1268 WB Mouse 1 : 200 Retina BV2 cell

Cholesterol Induces Pyroptosis and Matrix Degradation via mSREBP1-Driven Endoplasmic Reticulum Stress in Intervertebral Disc Degeneration.. Frontiers in Cell and Developmental Biology Front Cell Dev Biol. 2021 Jan;9:803132-803132 IF Rat 1:100 Nucleus pulposus (NP) cell

A Novel Ferroptosis-Based Molecular Signature Associated with Biochemical Recurrence-Free Survival and Tumor Immune Microenvironment of Prostate Cancer.. Frontiers in Cell and Developmental Biology Front Cell Dev Biol. 2021 Jan;9:774625-774625 IHC Human Prostate cancer (PCa) tissue，benign prostatic hyperplasia (BPH) tissue

CircINSR Regulates Fetal Bovine Muscle and Fat Development. Frontiers in Cell and Developmental Biology Front Cell Dev Biol. 2020; 8: 615638 IF Bovine 1:500 Myoblasts

Caspase-8 Inhibition Prevents the Cleavage and Degradation of E3 Ligase Substrate Receptor Cereblon and Potentiates Its Biological Function. Frontiers in Cell and Developmental Biology Front Cell Dev Biol. 2020 Dec;8:1562 WB Human RPMI8226 cell，MM1.S cell

Mechanism of deoxynivalenol-induced neurotoxicity in weaned piglets is linked to lipid peroxidation, dampened neurotransmitter levels, and interference with calcium signaling. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Ecotox Environ Safe. 2020 May;194:110382 WB Pig Brain

Degradation of GRK2 and AKT is an early and detrimental event in myocardial ischemia/reperfusion. EBioMedicine 2019 Oct 05 WB Rat cardiac

Hepatitis B Virus X Protein (HBx) Suppresses Transcription Factor EB (TFEB) Resulting in Stabilization of Integrin Beta 1 (ITGB1) in Hepatocellular Carcinoma Cells. Cancers Cancers. 2021 Jan;13(5):1181 WB Human HepG2 cell，SK-Hep-1 cell，HEK-293FT cell

Pilot Study of the Biological Properties and Vascularization of 3D Printed Bilayer Skin Grafts. International Journal of Bioprinting 2020; 6(1): 246 IHC Mouse 1: 500 skin

Roles of Crosstalk between Astrocytes and Microglia in Triggering Neuroinflammation and Brain Edema Formation in 1,2-Dichloroethane-Intoxicated Mice. Cells Cells-Basel. 2021 Oct;10(10):2647 WB Mouse 1:1000 Cerebral cortices

Circular RNA circMYL1 Inhibit Proliferation and Promote Differentiation of Myoblasts by Sponging miR-2400. Cells Cells-Basel. 2021 Jan;10(1):176 IF Bovine 1: 500 Myoblasts

C. tropicalis promotes chemotherapy resistance in colon cancer through increasing lactate production to regulate the mismatch repair system. International Journal of Biological Sciences Int J Biol Sci. 2021; 17(11): 2756–2769 WB Mouse SW480 cell-xenograft

MicroRNA-370 carried by M2 macrophage-derived exosomes alleviates asthma progression through inhibiting the FGF1/MAPK/STAT1 axis. International Journal of Biological Sciences Int J Biol Sci. 2021; 17(7): 1795–1807 WB Mouse Lung

Endogenous hydrogen sulfide alleviates methotrexate‐induced cognitive impairment by attenuating endoplasmic reticulum stress‐induced apoptosis via CHOP and caspase‐12. FUNDAMENTAL & CLINICAL PHARMACOLOGY Fund Clin Pharmacol. 2020 Oct;34(5):559-570 IHC Rat 1 : 100 hippocampus

MiR-487a Promotes TGF-β1-induced EMT, the Migration and Invasion of Breast Cancer Cells by Directly Targeting MAGI2. International Journal of Biological Sciences Int J Biol Sci. 2016; 12(4): 397–408 WB Human 1:1000 MCF-7 cell, MDA-MB-231 cell

Design and application of hydrophilic bimetallic metal-organic framework magnetic nanoparticles for rapid capture of exosomes. ANALYTICA CHIMICA ACTA Anal Chim Acta. 2021 Nov;1186:339099 WB Human Urinary exosomes HeLa cells

Protective effect of thioredoxin reductase 1 in Parkinson's disease. NEUROSCIENCE LETTERS Neurosci Lett. 2021 Jan;741:135457 IHC,IF Mouse Brain

Sustained high expression of NRF2 and its target genes induces dysregulation of cellular proliferation and apoptosis is associated with arsenite-induced malignant transformation of human bronchial epithelial cells. SCIENCE OF THE TOTAL ENVIRONMENT Sci Total Environ. 2021 Feb;756:143840 WB Human Human bronchial epithelial(HBE)

Akt inhibition-dependent downregulation of the Wnt/β-Catenin Signaling pathway contributes to antimony-induced neurotoxicity. SCIENCE OF THE TOTAL ENVIRONMENT Sci Total Environ. 2020 Oct;737:140252 IHC Mouse 1:400 Brain

Ficus pandurata Hance Inhibits Ulcerative Colitis and Colitis-Associated Secondary Liver Damage of Mice by Enhancing Antioxidation Activity. Oxidative Medicine and Cellular Longevity Oxid Med Cell Longev. 2021;2021:2617881 WB Mouse Colon

miR-378 inhibits cell growth and enhances apoptosis in human myelodysplastic syndromes. INTERNATIONAL JOURNAL OF ONCOLOGY 2016 Nov 01 WB Human SKM-1 cell

Pb induced mitochondrial fission of fibroblast cells via ATM activation. JOURNAL OF HAZARDOUS MATERIALS J Hazard Mater. 2021 Aug;416:126177 WB Human 1 : 1000 GM00637 cell

Aucubin Alleviates Seizures Activity in Li-Pilocarpine-Induced Epileptic Mice: Involvement of Inhibition of Neuroinflammation and Regulation of Neurotransmission. NEUROCHEMICAL RESEARCH Neurochem Res. 2019 Feb;44(2):472-484 WB Mouse 1:500 hippocampus

Polygonum cuspidatum Extract Exerts Antihyperlipidemic Effects by Regulation of PI3K/AKT/FOXO3 Signaling Pathway. Oxidative Medicine and Cellular Longevity Oxid Med Cell Longev. 2021;2021:3830671 IF,WB Human 1:1000 HepG2 cells

TXNRD1: A Key Regulator Involved in the Ferroptosis of CML Cells Induced by Cysteine Depletion In Vitro. Oxidative Medicine and Cellular Longevity Oxid Med Cell Longev. 2021;2021:7674565 WB Human K562 cell

The Antitriple Negative Breast cancer Efficacy of Spatholobus suberectus Dunn on ROS-Induced Noncanonical Inflammasome Pyroptotic Pathway. Oxidative Medicine and Cellular Longevity Oxid Med Cell Longev. 2021;2021:5187569 WB Human MDA-MB-231 cells

Involvement of Hepcidin in Cognitive Damage Induced by Chronic Intermittent Hypoxia in Mice. Oxidative Medicine and Cellular Longevity Oxid Med Cell Longev. 2021;2021:8520967 WB Mouse 1:2000 Hippocampus

Effects of Fluorine on Neutrophil Extracellular Trap Formation through Regulating AMPK/p38 Signaling Pathway. Oxidative Medicine and Cellular Longevity Oxid Med Cell Longev. 2021;2021:6693921 WB Fish 1:10000 Neutrophils

Mangiferin improves hepatic damage-associated molecular patterns, lipid metabolic disorder and mitochondrial dysfunction in alcohol hepatitis rats. Food & Function Food Funct. 2019 Jun;10(6):3514-3534 IHC Rat Liver, kidney , spleen

Bu Shen Yi Sui Capsule Alleviates Neuroinflammation and Demyelination by Promoting Microglia toward M2 Polarization, Which Correlates with Changes in miR-124 and miR-155 in Experimental Autoimmune Encephalomyelitis. Oxidative Medicine and Cellular Longevity Oxid Med Cell Longev. 2021;2021:5521503 WB Mouse 1 : 1000 Myocardium

Bixin Attenuates High-Fat Diet-Caused Liver Steatosis and Inflammatory Injury through Nrf2/PPARα Signals. Oxidative Medicine and Cellular Longevity Oxid Med Cell Longev. 2021;2021:6610124 IF Human LO2 cell

CD38 enhances the proliferation and inhibits the apoptosis of cervical cancer cells by affecting the mitochondria functions. MOLECULAR CARCINOGENESIS Mol Carcinogen. 2017 Oct;56(10):2245-2257 WB Human,Mouse 1:1000 CaSki cell-xenograft,cervical cancer tissues CaSki cell， HeLa cell

Inhibition of store‑operated Ca2+ entry counteracts the apoptosis of nasopharyngeal carcinoma cells induced by sodium butyrate. Oncology Letters Oncol Lett. 2017 Feb;13(2):921-929 WB Human 5–8F cell,6–10B cell

Hydroxy-α-sanshool Possesses Protective Potentials on H2O2-Stimulated PC12 Cells by Suppression of Oxidative Stress-Induced Apoptosis through Regulation of PI3K/Akt Signal Pathway. Oxidative Medicine and Cellular Longevity Oxid Med Cell Longev. 2020;2020:3481758 WB Rat 1 : 1000 PC12 cell

Protective effects of a novel probiotic strain, Lactococcus lactis ML2018, in colitis: in vivo and in vitro evidence. Food & Function Food Funct. 2019 Feb;10(2):1132-1145 WB Mouse 1:50 colon tissues

Preventive effect of sanguinarine on intestinal injury in mice exposed to whole abdominal irradiation. BIOMEDICINE & PHARMACOTHERAPY Biomed Pharmacother. 2022 Feb;146:112496 WB Rat IEC-6 cell

Cajanolactone A, a stilbenoid from Cajanus cajan, inhibits energy intake and lipid synthesis/storage, and promotes energy expenditure in ovariectomized mice. BIOMEDICINE & PHARMACOTHERAPY Biomed Pharmacother. 2021 Jun;138:111491 WB Mouse 1:10000 Hypothalami, BATs, iWATs, pWATs, liver

Gu-Ben-Fang-Xiao decoction modulates lipid metabolism by activating the AMPK pathway in asthma remission. BIOMEDICINE & PHARMACOTHERAPY Biomed Pharmacother. 2021 Jun;138:111403 WB Mouse 1:1000 Lung

Tumor-Associated Fibroblast-Targeted Regulation and Deep Tumor Delivery of Chemotherapeutic Drugs with a Multifunctional Size-Switchable Nanoparticle. ACS Applied Materials & Interfaces Acs Appl Mater Inter. 2019;11(43):39545–39559 IF Mouse 1:100 4T1 tumor

Rg1 Attenuates alcoholic hepatic damage through regulating AMP-activated protein kinase and nuclear factor erythroid 2-related factor 2 signal pathways. JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH 2016 May 26 WB Mouse liver

PTH1-34 promotes osteoblast formation through Beclin1-dependent autophagic activation. JOURNAL OF BONE AND MINERAL METABOLISM J Bone Miner Metab. 2021 Jul;39(4):572-582 WB Mouse MC3T3-E1 cell

Telekin suppresses human hepatocellular carcinoma cells in vitro by inducing G 2 /M phase arrest via the p38 MAPK signaling pathway. ACTA PHARMACOLOGICA SINICA Acta Pharmacol Sin. 2014 Sep;35(10):1311-1322 WB Human HepG2 cell

Paclitaxel increases sensitivity of SKOV3 cells to hyperthermia by inhibiting heat shock protein 27. BIOMEDICINE & PHARMACOTHERAPY Biomed Pharmacother. 2020 Dec;132:110907 WB Human SKOV3 cell

Gu-Ben-Fang-Xiao attenuates allergic airway inflammation by inhibiting BAFF-mediated B cell activation. BIOMEDICINE & PHARMACOTHERAPY Biomed Pharmacother. 2020 Dec;132:110801 WB Mouse 1 : 1000 Lung，spleen

Down-regulation of FoxM1 by thiostrepton or small interfering RNA inhibits proliferation, transformation ability and angiogenesis, and induces apoptosis of nasopharyngeal carcinoma cells. International Journal of Clinical and Experimental Pathology Int J Clin Exp Patho. 2014; 7(9): 5450–5460 WB Human Nasopharyngeal carcinoma (NPC) cell

α1-nAchR-Mediated Signaling Through Lipid Raft Is Required for Nicotine-Induced NLRP3 Inflammasome Activation and Nicotine-Accelerated Atherosclerosis. Frontiers in Cell and Developmental Biology Front Cell Dev Biol. 2021 Aug;0:2161 IHC Mouse Aortic root

Ganoderic Acids Prevent Renal Ischemia Reperfusion Injury by Inhibiting Inflammation and Apoptosis. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Int J Mol Sci. 2021 Jan;22(19):10229 WB Rat Renal tissues NRK-52E cell

Serum proteomics analysis based on label-free revealed the protective effect of Chinese herbal formula Gu-Ben-Fang-Xiao. BIOMEDICINE & PHARMACOTHERAPY Biomed Pharmacother. 2019 Nov;119:109390 WB Mouse 1:1000 lung tissue

miR-378 inhibits cell growth and enhances apoptosis in human myelodysplastic syndromes. INTERNATIONAL JOURNAL OF ONCOLOGY 2016 Nov 01 WB Human SKM-1 cell

Danggui-Shaoyao-San (DSS) Ameliorates Cerebral Ischemia-Reperfusion Injury via Activating SIRT1 Signaling and Inhibiting NADPH Oxidases. Frontiers in Pharmacology Front Pharmacol. 2021 Apr;0:747 IHC Rat 1 : 100 Brain

Rce1 expression in renal cell carcinoma and its regulatory effect on 786-O cell apoptosis through endoplasmic reticulum stress. ACTA BIOCHIMICA ET BIOPHYSICA SINICA 2017 Mar 01 WB Human 1:2000 renal cancer tissues 786-O cell

Insulin regulates primordial-follicle assembly in vitro by affecting germ-cell apoptosis and elevating oestrogen. REPRODUCTION FERTILITY AND DEVELOPMENT Reprod Fert Develop. 2015 Oct;27(8):1197-1204 WB Mouse ovaries

CXCL5, the upregulated chemokine in patients with uterine cervix cancer, in vivo and in vitro contributes to oncogenic potential of Hela uterine cervix cancer cells. BIOMEDICINE & PHARMACOTHERAPY Biomed Pharmacother. 2018 Nov;107:1496 WB Human,Mouse 1:1000 Hela cell-xenografts Hela cell

p62/SQSTM1 accumulation due to degradation inhibition and transcriptional activation plays a critical role in silica nanoparticle-induced airway inflammation via NF-κB activation. JOURNAL OF NANOBIOTECHNOLOGY J Nanobiotechnol. 2020 Dec;18(1):1-15 WB,IF,IHC Mouse，Human lung BEAS-2b cell

Centromere protein U (CENPU) enhances angiogenesis in triple-negative breast cancer by inhibiting ubiquitin–proteasomal degradation of COX-2. CANCER LETTERS 2019 Nov 06 ELISA Mouse Plasma

Centromere protein U (CENPU) enhances angiogenesis in triple-negative breast cancer by inhibiting ubiquitin–proteasomal degradation of COX-2. CANCER LETTERS 2019 Nov 06 WB,IHC Human 1:2000,1:250 Breast cancer tissues Cal51 cell, MDA-MB-231 cell

Centromere protein U (CENPU) enhances angiogenesis in triple-negative breast cancer by inhibiting ubiquitin–proteasomal degradation of COX-2. CANCER LETTERS 2019 Nov 06 WB,IHC Human 1:2000，1：250 Breast cancer tissues Cal51 cell, MDA-MB-231 cell

Centromere protein U (CENPU) enhances angiogenesis in triple-negative breast cancer by inhibiting ubiquitin–proteasomal degradation of COX-2. CANCER LETTERS 2019 Nov 06 WB Human 1:1000，1：300 Breast cancer tissues Cal51 cell, MDA-MB-231 cell

SERPINE2 promotes esophageal squamous cell carcinoma metastasis by activating BMP4. CANCER LETTERS Cancer Lett. 2020 Jan;469:390 WB Human KYSE30 cell, KYSE450 cell

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Western Blot analysis of various cells using Smad1/5/9 Polyclonal Antibody diluted at 1:500

Western blot analysis of lysates from HeLa cells, using Smad1/5/9 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).