PYK2 Polyclonal Antibody

    • Catalog No.:YT3912
    • Applications:WB,IHC-p,ELISA
    • Reactivity:Human,Mouse,Rat,Monkey
      • Gene Name:
      • PTK2B
      • Protein Name:
      • Protein-tyrosine kinase 2-beta
      • Human Gene Id:
      • 2185
      • Human Swiss Prot No:
      • Q14289
      • Mouse Swiss Prot No:
      • Q9QVP9
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human PYK2. AA range:546-595
      • Specificity:
      • PYK2 Polyclonal Antibody detects endogenous levels of PYK2 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • PTK2B; FAK2; PYK2; RAFTK; Protein-tyrosine kinase 2-beta; Calcium-dependent tyrosine kinase; CADTK; Calcium-regulated non-receptor proline-rich tyrosine kinase; Cell adhesion kinase beta; CAK-beta; CAKB; Focal adhesion kinase 2; FADK 2; Pro
      • MolecularWeight(Da):
      • 115875
      • Observed Band(KD):
      • 115
      • Background:
      • protein tyrosine kinase 2 beta(PTK2B) Homo sapiens This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity t
      • Function:
      • catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,function:Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation.,PTM:Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration, as well as by PKC activation. Recruitment by nephrocystin to cell matrix adhesions initiates Tyr-402
      • Subcellular Location:
      • nucleus,nucleoplasm,cytoplasm,cytosol,cytoskeleton,focal adhesion,cell cortex,postsynaptic density,NMDA selective glutamate receptor complex,lamellipodium,axon,dendrite,growth cone,extrinsic compone
      • Expression:
      • Brain,Hippocampus,Lymph,Monocyte,Platelet,Testis,
      • Products Images
      • Western Blot analysis of various cells using PYK2 Polyclonal Antibody
      • Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
      • Immunohistochemistry analysis of paraffin-embedded human brain tissue, using PYK2 Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from COS cells, treated with UV 15', using PYK2 Antibody. The lane on the right is blocked with the synthesized peptide.