PRAS40 (Phospho Ser183) rabbit pAb
- Catalog No.:YP1449
- Applications:WB;ELISA;IHC
- Reactivity:Human;Mouse
- Target:
- PRAS40
- Fields:
- >>Autophagy - animal;>>mTOR signaling pathway;>>AMPK signaling pathway;>>Longevity regulating pathway;>>Longevity regulating pathway - multiple species;>>Thermogenesis;>>Shigellosis
- Gene Name:
- AKT1S1 PRAS40
- Protein Name:
- PRAS40 (Ser183)
- Human Gene Id:
- 84335
- Human Swiss Prot No:
- Q96B36
- Mouse Gene Id:
- 67605
- Mouse Swiss Prot No:
- Q9D1F4
- Immunogen:
- Synthesized phosho peptide around human PRAS40 (Ser183)
- Specificity:
- This antibody detects endogenous levels of Human Mouse PRAS40 (phospho-Ser183)
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
- Purification:
- The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- Proline-rich AKT1 substrate 1 (40 kDa proline-rich AKT substrate)
- Observed Band(KD):
- 40kD
- Background:
- AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM, Mar 2008],
- Function:
- function:May play an important role in phosphatidylinositol 3-kinase (PI3K)-AKT1 survival signaling. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. Its role in survival signaling pathways may be modulated by oxidative stress. May also play a role in nerve growth factor-mediated neuroprotection.,subcellular location:Found in the cytosolic fraction of the brain.,subunit:The phosphorylated form interacts with 14-3-3.,tissue specificity:Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A549 and HeLa) than in normal cell lines (e.g. HEK293).,
- Subcellular Location:
- Cytoplasm, cytosol . Found in the cytosolic fraction of the brain. .
- Expression:
- Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A-549 and HeLa) than in normal cell lines (e.g. HEK293).
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- Antibody-FAQs
- Products Images
- Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night
- Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).