• Target：
• PRAS40

• Fields：
• >>Autophagy - animal;>>mTOR signaling pathway;>>AMPK signaling pathway;>>Longevity regulating pathway;>>Longevity regulating pathway - multiple species;>>Thermogenesis;>>Shigellosis

• Gene Name：
• AKT1S1

• Protein Name：
• Proline-rich AKT1 substrate 1

• Human Gene Id：
• 84335

• Human Swiss Prot No：
• Q96B36

• Mouse Gene Id：
• 67605

• Mouse Swiss Prot No：
• Q9D1F4

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human Akt1 S1 around the phosphorylation site of Thr246. AA range:207-256

• Specificity：
• Phospho-PRAS40 (T246) Polyclonal Antibody detects endogenous levels of PRAS40 protein only when phosphorylated at T246.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. ELISA: 1:10000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• AKT1S1;PRAS40;Proline-rich AKT1 substrate 1;40 kDa proline-rich AKT substrate

• Observed Band(KD)：
• 40kD

• Background：
• AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM, Mar 2008],

• Function：
• function:May play an important role in phosphatidylinositol 3-kinase (PI3K)-AKT1 survival signaling. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. Its role in survival signaling pathways may be modulated by oxidative stress. May also play a role in nerve growth factor-mediated neuroprotection.,subcellular location:Found in the cytosolic fraction of the brain.,subunit:The phosphorylated form interacts with 14-3-3.,tissue specificity:Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A549 and HeLa) than in normal cell lines (e.g. HEK293).,

• Subcellular Location：
• Cytoplasm, cytosol . Found in the cytosolic fraction of the brain. .

• Expression：
• Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A-549 and HeLa) than in normal cell lines (e.g. HEK293).

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