Tuberin (phospho Thr1462) Polyclonal Antibody

    • Catalog No.:YP0968
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • Tuberin
      • Fields:
      • >>Phospholipase D signaling pathway;>>p53 signaling pathway;>>Autophagy - animal;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>AMPK signaling pathway;>>Longevity regulating pathway;>>Cellular senescence;>>Thermogenesis;>>Insulin signaling pathway;>>Thyroid hormone signaling pathway;>>Human cytomegalovirus infection;>>Human papillomavirus infection;>>Herpes simplex virus 1 infection;>>Choline metabolism in cancer
      • Gene Name:
      • TSC2
      • Protein Name:
      • Tuberin
      • Human Gene Id:
      • 7249
      • Human Swiss Prot No:
      • P49815
      • Mouse Swiss Prot No:
      • Q61037
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human Tuberin/TSC2 around the phosphorylation site of Thr1462. AA range:1428-1477
      • Specificity:
      • Phospho-Tuberin (T1462) Polyclonal Antibody detects endogenous levels of Tuberin protein only when phosphorylated at T1462.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500-2000 IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • TSC2;TSC4;Tuberin;Tuberous sclerosis 2 protein
      • Observed Band(KD):
      • 200kD
      • Background:
      • Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],
      • Function:
      • alternative products:Additional isoforms seem to exist. Experimental confirmation may be lacking for some isoforms,disease:Defects in TSC2 are a cause of lymphangioleiomyomatosis (LAM) [MIM:606690]. LAM is a progressive and often fatal lung disease characterized by a diffuse proliferation of abnormal smooth muscle cells in the lungs. It affects almost exclusively young women and can occur as an isolated disorder or in association with tuberous sclerosis complex.,disease:Defects in TSC2 are the cause of tuberous sclerosis complex (TSC) [MIM:191100]. The molecular basis of TSC is a functional impairment of the tuberin-hamartin complex. TSC is an autosomal dominant multi-system disorder that affects especially the brain, kidneys, heart, and skin. TSC is characterized by hamartomas (benign overgrowths predominantly of a cell or tissue type that occurs normally in the organ) and hamartias (de
      • Subcellular Location:
      • Cytoplasm. Membrane; Peripheral membrane protein. At steady state found in association with membranes.
      • Expression:
      • Liver, brain, heart, lymphocytes, fibroblasts, biliary epithelium, pancreas, skeletal muscle, kidney, lung and placenta.
      • Products Images
      • Western blot analysis of 293 using p-Tuberin (T1462) antibody.
      • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Tuberin/TSC2 (Phospho-Thr1462) Antibody
      • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Tuberin/TSC2 (Phospho-Thr1462) Antibody. The picture on the right is blocked with the phospho peptide.