TRAP220 (phospho Thr1457) Polyclonal Antibody

    • Catalog No.:YP0890
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse;Monkey
      • Target:
      • TRAP220
      • Fields:
      • >>Endocrine resistance;>>Thyroid hormone signaling pathway
      • Gene Name:
      • MED1
      • Protein Name:
      • Mediator of RNA polymerase II transcription subunit 1
      • Human Gene Id:
      • 5469
      • Human Swiss Prot No:
      • Q15648
      • Mouse Swiss Prot No:
      • Q925J9
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human PPAR-BP around the phosphorylation site of Thr1457. AA range:1423-1472
      • Specificity:
      • Phospho-TRAP220 (T1457) Polyclonal Antibody detects endogenous levels of TRAP220 protein only when phosphorylated at T1457.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • MED1;ARC205;CRSP1;CRSP200;DRIP205;DRIP230;PBP;PPARBP;PPARGBP;RB18A;TRAP220;TRIP2;Mediator of RNA polymerase II transcription subunit 1;Activator-recruited cofactor 205 kDa component;ARC205;Mediator complex subunit 1;Peroxiso
      • Observed Band(KD):
      • 168kD
      • Background:
      • The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. It also regulates p53-dependent apoptosis and it is essential for adipogenesis. This protein is known to have the ability to self-oligomerize. [provided by RefSeq, Jul 2008],
      • Function:
      • function:Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.,PTM:Phosphorylated by MAPK1 or MAPK3 during G2/M phase which may enhance protein stability and promote entry into the nucleolus. Phosphorylated upon DNA damage, probably by ATM or ATR.,sequence caution:Contaminating sequence. Potential poly-A sequence.,similarity:Belongs to the Mediator complex subunit 1 family.,subcellular location:A subset of the protein may enter the nucleol
      • Subcellular Location:
      • Nucleus . A subset of the protein may enter the nucleolus subsequent to phosphorylation by MAPK1 or MAPK3.
      • Expression:
      • Ubiquitously expressed.
      • Products Images
      • Immunofluorescence analysis of HeLa cells, using PPAR-BP (Phospho-Thr1457) Antibody. The picture on the right is blocked with the phospho peptide.
      • Immunohistochemistry analysis of paraffin-embedded human heart, using PPAR-BP (Phospho-Thr1457) Antibody. The picture on the right is blocked with the phospho peptide.
      • Western blot analysis of lysates from HUVEC cells treated with Serum 20% 30', using PPAR-BP (Phospho-Thr1457) Antibody. The lane on the right is blocked with the phospho peptide.