• Target：
• RFA2

• Fields：
• >>DNA replication;>>Nucleotide excision repair;>>Mismatch repair;>>Homologous recombination;>>Fanconi anemia pathway

• Gene Name：
• RPA2

• Protein Name：
• Replication protein A 32 kDa subunit

• Human Gene Id：
• 6118

• Human Swiss Prot No：
• P15927

• Mouse Swiss Prot No：
• Q62193

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human RFA2 around the phosphorylation site of Thr21. AA range:10-59

• Specificity：
• Phospho-RPA32 (T21) Polyclonal Antibody detects endogenous levels of RPA32 protein only when phosphorylated at T21.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• RPA2;REPA2;RPA32;RPA34;Replication protein A 32 kDa subunit;RP-A p32;Replication factor A protein 2;RF-A protein 2;Replication protein A 34 kDa subunit;RP-A p34

• Observed Band(KD)：
• 35kD

• Background：
• function:Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.,PTM:Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDC2.,subcellular location:Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.,subunit:Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN.,

• Function：
• function:Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.,PTM:Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDC2.,subcellular location:Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.,subunit:Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN.,

• Subcellular Location：
• Nucleus . Nucleus, PML body . Redistributes to discrete nuclear foci upon DNA damage in an ATR-dependent manner. .

• Expression：
• Kidney,Lung,Muscle,

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