• Target：
• PFK-2 car

• Fields：
• >>Fructose and mannose metabolism;>>Metabolic pathways;>>AMPK signaling pathway;>>Thyroid hormone signaling pathway

• Gene Name：
• PFKFB2

• Protein Name：
• 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2

• Human Gene Id：
• 5208

• Human Swiss Prot No：
• O60825

• Mouse Gene Id：
• 18640

• Mouse Swiss Prot No：
• P70265

• Rat Gene Id：
• 24640

• Rat Swiss Prot No：
• Q9JJH5

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human PFKFB2 around the phosphorylation site of Ser483. AA range:451-500

• Specificity：
• Phospho-PFK-2 car (S483) Polyclonal Antibody detects endogenous levels of PFK-2 car protein only when phosphorylated at S483.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• PFKFB2;6-phosphofructo-2-kinase/fructose-2;6-bisphosphatase 2;6PF-2-K/Fru-2,6-P2ase 2;PFK/FBPase 2;6PF-2-K/Fru-2,6-P2ase heart-type isozyme

• Observed Band(KD)：
• 58kD

• Background：
• The protein encoded by this gene is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. This protein regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

• Function：
• catalytic activity:ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.,catalytic activity:Beta-D-fructose 2,6-bisphosphate + H(2)O = D-fructose 6-phosphate + phosphate.,enzyme regulation:Phosphorylation results in the activation of the kinase activity.,function:Synthesis and degradation of fructose 2,6-bisphosphate.,similarity:In the C-terminal section; belongs to the phosphoglycerate mutase family.,subunit:Homodimer.,tissue specificity:Heart.,

• Subcellular Location：
• cytosol,

• Expression：
• Heart.

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