Ret (phospho Tyr1062) Polyclonal Antibody

    • Catalog No.:YP0519
    • Applications:WB,IHC-p,IF(paraffin section),ELISA
    • Reactivity:Human,Mouse,Rat
      • Gene Name:
      • RET
      • Protein Name:
      • Proto-oncogene tyrosine-protein kinase receptor Ret
      • Human Gene Id:
      • 5979
      • Human Swiss Prot No:
      • P07949
      • Mouse Swiss Prot No:
      • P35546
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human Ret around the phosphorylation site of Tyr1062. AA range:1041-1090
      • Specificity:
      • Phospho-Ret (Y1062) Polyclonal Antibody detects endogenous levels of Ret protein only when phosphorylated at Y1062.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • IF(paraffin section) 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • RET; CDHF12; CDHR16; PTC; RET51; Proto-oncogene tyrosine-protein kinase receptor Ret; Cadherin family member 12; Proto-oncogene c-Ret
      • MolecularWeight(Da):
      • 124319
      • Observed Band(KD):
      • 170
      • Background:
      • ret proto-oncogene(RET) Homo sapiens This gene, a member of the cadherin superfamily, encodes one of the receptor tyrosine kinases, which are cell-surface molecules that transduce signals for cell growth and differentiation. This gene plays a crucial role in neural crest development, and it can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement. Mutations in this gene are associated with the disorders multiple endocrine neoplasia, type IIA, multiple endocrine neoplasia, type IIB, Hirschsprung disease, and medullary thyroid carcinoma. Two transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their biological validity has not been confirmed. [provided by RefSeq, Jul 2008],
      • Function:
      • catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,disease:Chromosomal aberrations involving RET are a cause of thyroid papillary carcinoma (PACT) [MIM:188550]. Inversion inv(10)(q11.2;q21) generates the RET/CCDC6 (PTC1) oncogene; inversion inv(10)(q11.2;q11.2) generates the RET/NCOA4 (PTC3) oncogene; translocation t(10;14)(q11;q32) with GOLGA5 generates the RET/GOLGA5 (PTC5) oncogene; translocation t(8;10)(p21.3;q11.2) with PCM1 generates the PCM1/RET fusion; translocation t(6;10)(p21.3;q11.2) with RFP generates the Delta RFP/RET oncogene; translocation t(1;10)(p13;q11) with TRIM33 generates the TRIM33/RET (PTC7) oncogene; translocation t(7;10)(q32;q11) with TIF1 generates the TIF1/RET (PTC6) oncogene. The PTC5 oncogene has been found in 2 cases of PACT in children exposed to radioactive fallout after Chernobyl.,disease:Defects in RET are a cause o
      • Subcellular Location:
      • cytoplasm,early endosome,plasma membrane,integral component of plasma membrane,endosome membrane,membrane,integral component of membrane,axon,dendrite,neuronal cell body,intracellular membrane-bounded organelle,receptor complex,
      • Expression:
      • Blood,Brain,Fibroblast,Leukocyte,Neural crest,Peripheral blood leukocyte,Thyroid papillary
      • Products Images
      • Western blot analysis of Ret (phospho Tyr1062) Polyclonal Antibody, using HepG2 cell treated or untreated with forskolin 40nM 30', 4° over night, secondary antibody(cat: RS0002 was diluted at 1:10000, 37° 1hour.
      • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Ret (Phospho-Tyr1062) Antibody
      • Western blot analysis of lysates from K562 cells, using Ret (Phospho-Tyr1062) Antibody. The lane on the right is blocked with the phospho peptide.