• Target：
• PKA IIβ reg

• Fields：
• >>Insulin signaling pathway

• Gene Name：
• PRKAR2B

• Protein Name：
• cAMP-dependent protein kinase type II-beta regulatory subunit

• Human Gene Id：
• 5577

• Human Swiss Prot No：
• P31323

• Mouse Gene Id：
• 19088

• Mouse Swiss Prot No：
• P31324

• Rat Gene Id：
• 24679

• Rat Swiss Prot No：
• P12369

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human PKA-R2 beta around the phosphorylation site of Ser113. AA range:79-128

• Specificity：
• Phospho-PKA IIβ reg (S113) Polyclonal Antibody detects endogenous levels of PKA IIβ reg protein only when phosphorylated at S113.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• PRKAR2B;cAMP-dependent protein kinase type II-beta regulatory subunit

• Observed Band(KD)：
• 46kD

• Background：
• cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activ

• Function：
• function:Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.,PTM:Phosphorylated by the activated catalytic chain.,similarity:Belongs to the cAMP-dependent kinase regulatory chain family.,similarity:Contains 2 cyclic nucleotide-binding domains.,subunit:The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules.,tissue specificity:Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.,

• Subcellular Location：
• Cytoplasm . Cell membrane . Colocalizes with PJA2 in the cytoplasm and at the cell membrane.

• Expression：
• Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

IL-37 suppresses hepatocellular carcinoma growth by converting pSmad3 signaling from JNK/pSmad3L/c-Myc oncogenic signaling to pSmad3C/P21 tumor-suppressive signaling. Oncotarget Oncotarget. 2016 Dec 20; 7(51): 85079–85096 WB,IHC Human 1:250 HCC tissue SMMC-7721 cell

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