PR (phospho Ser190) Polyclonal Antibody

    • Catalog No.:YP0235
    • Applications:WB,IHC-p,IF(paraffin section),ELISA
    • Reactivity:Human,Monkey
      • Gene Name:
      • PGR
      • Protein Name:
      • Progesterone receptor
      • Human Gene Id:
      • 5241
      • Human Swiss Prot No:
      • P06401
      • Mouse Swiss Prot No:
      • Q00175
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human Progesterone Receptor around the phosphorylation site of Ser190. AA range:161-210
      • Specificity:
      • Phospho-PR (S190) Polyclonal Antibody detects endogenous levels of PR protein only when phosphorylated at S190.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • IF(paraffin section) 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • PGR; NR3C3; Progesterone receptor; PR; Nuclear receptor subfamily 3 group C member 3
      • MolecularWeight(Da):
      • 98981
      • Observed Band(KD):
      • 99
      • Background:
      • progesterone receptor(PGR) Homo sapiens This gene encodes a member of the steroid receptor superfamily. The encoded protein mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce several transcript variants, both protein coding and non-protein coding. Two of the isoforms (A and B) are identical except for an additional 165 amino acids found in the N-terminus of isoform B and mediate their own response genes and physiologic effects with little overlap. [provided by RefSeq, Sep 2015],
      • Function:
      • domain:Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain.,function:Isoform A is inactive in stimulating c-Src/MAPK signaling on hormone stimulation.,function:The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.,online information:Progesterone receptor entry,PTM:Phosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Se
      • Subcellular Location:
      • nucleus,nucleoplasm,mitochondrial outer membrane,
      • Expression:
      • Breast cancer,Platelet,Sperm,
      • Products Images
      • Western Blot analysis of various cells using Phospho-PR (S190) Polyclonal Antibody diluted at 1:1000
      • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Progesterone Receptor (Phospho-Ser190) Antibody.
      • Western blot analysis of lysates from COS7 cells treated with EGF, using Progesterone Receptor (Phospho-Ser190) Antibody. The lane on the right is blocked with the phospho peptide.