Survivin Monoclonal Antibody(Mix)

    • Catalog No.:YM3419
    • Applications:WB,IHC-p
    • Reactivity:Human,Rat
      • Gene Name:
      • BIRC5
      • Protein Name:
      • Baculoviral IAP repeat-containing protein 5
      • Human Gene Id:
      • 332
      • Human Swiss Prot No:
      • O15392
      • Mouse Swiss Prot No:
      • O70201
      • Immunogen:
      • Recombinant Protein of Survivin
      • Specificity:
      • The antibody detects endogenous Survivin protein.
      • Formulation:
      • PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
      • Source:
      • Mouse
      • Dilution:
      • WB: 1:1000-2000 IHC: 1:200-500
      • Purification:
      • The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • BIRC5; API4; IAP4; Baculoviral IAP repeat-containing protein 5; Apoptosis inhibitor 4; Apoptosis inhibitor survivin
      • Observed Band(KD):
      • 16
      • Background:
      • baculoviral IAP repeat containing 5(BIRC5) Homo sapiens This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors, yet low in adult tissues. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jun 2011],
      • Function:
      • domain:The BIR repeat is necessary and sufficient for HBXIP binding.,function:May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Interacts with tubulin. Inhibitor of caspase-3 and caspase-7. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Isoforms 2 and 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.,similarity:Belongs to the IAP family.,similarity:Contains 1 BIR repeat.,subcellular location:Localizes on chromosome arms and inner centromeres from prophase through metaphase and t
      • Subcellular Location:
      • nuclear chromosome,chromosome, centromeric region,condensed chromosome kinetochore,nucleus,nucleoplasm,cytoplasm,centriole,spindle,cytosol,microtubule,spindle microtubule,cytoplasmic microtubule,midbod
      • Expression:
      • Epithelium,Eye,Lung,Mammary cancer,Mammary gland,Muscle,Myeloid leu
      • Products Images
      • Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1,Survivin Monoclonal Antibody(Mix) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Rat-spinal-cord tissue. 1,Survivin Monoclonal Antibody(Mix) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue. 1,Survivin Monoclonal Antibody(Mix) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Western blot analysis of 1) Hela, 2) 293, 3) PC12 using Survivin Monoclonal Antibody.
      • Immunohistochemical analysis of paraffin-embedded human colon caricnoma using Survivin Monoclonal Antibody.
      • Western blot detection of Survivin in human breast cancer cell line MCF-7(A) and Cal51 (B) using Survivin mouse mAb (YM3419, 1:1000 diluted).Predicted band size: 16kDa.Observed band size:16kDa. Picture was kindly provided by our customer from Tianjin Medical University Cancer Institute and Hospital
      • Immunohistochemical analysis of paraffin-embedded Human breast cancer. 1, Using Survivin Mouse mAb (YM3419) diluted at 1:200 (4°,overnight). 2, High-pressure and temperature Citric acid, pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 50min). Picture was kindly provided by our customer from Tianjin Medical University Cancer Institute and Hospital
      • Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
      • Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
      • Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).