PCNA Monoclonal Antibody(12D10)

    • Catalog No.:YM3031
    • Applications:WB;IF;IHC
    • Reactivity:Human;Rat;Mouse
      • Target:
      • PCNA
      • Fields:
      • >>DNA replication;>>Base excision repair;>>Nucleotide excision repair;>>Mismatch repair;>>Cell cycle;>>Tight junction;>>Hepatitis B
      • Gene Name:
      • PCNA
      • Protein Name:
      • Proliferating cell nuclear antigen
      • Human Gene Id:
      • 5111
      • Human Swiss Prot No:
      • P12004
      • Mouse Swiss Prot No:
      • P17918
      • Immunogen:
      • Synthetic Peptide of PCNA
      • Specificity:
      • The antibody detects endogenous PCNA protein.
      • Formulation:
      • PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
      • Source:
      • Monoclonal, Mouse
      • Dilution:
      • WB 1:5000 IHC 1:200 IF 1:200
      • Purification:
      • The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • PCNA;Proliferating cell nuclear antigen;PCNA;Cyclin
      • Observed Band(KD):
      • 30-33kd
      • Background:
      • The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008],
      • Function:
      • disease:Antibodies are present in sera from patients with systemic lupus erythematosus.,function:This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.,online information:PCNA entry,PTM:Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical poly-ubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases RNF8 and SHPRH, which are required for DNA repair.,similarity:Belongs to the PCNA family.,subunit:Homotrimer. Interacts with KCTD10. Interacts with PPP1R15A (By similarity). Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with POLH, POLK, DNMT1, ERCC5/XPG, FEN1, C
      • Subcellular Location:
      • Nucleus . Colocalizes with CREBBP, EP300 and POLD1 to sites of DNA damage (PubMed:24939902). Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase (PubMed:15543136). Co-localizes with SMARCA5/SNF2H and BAZ1B/WSTF at replication foci during S phase (PubMed:15543136). Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. .
      • Expression:
      • Bone marrow,Fetal brain cortex,Lung,Placenta,
      • Products Images
      • Immunofluorescence analysis of Hela cell. 1,14-3-3 θ/τ (phospho Ser232) Polyclonal Antibody(green) was diluted at 1:200(4° overnight). (red) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog:RS3608 was diluted at 1:1000(room temperature, 50min).
      • Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,PCNA Monoclonal Antibody(12D10) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,PCNA Monoclonal Antibody(12D10) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,PCNA Monoclonal Antibody(12D10) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunofluorescence analysis of Human-lung-cancer tissue. 1,PCNA Monoclonal Antibody(12D10)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
      • Immunofluorescence analysis of Rat-testis tissue. 1,PCNA Monoclonal Antibody(12D10)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
      • Western blot analysis of Hela (1), Rat brain (2), NIH 3T3 (3), 293T (4), diluted at 1:5000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
      • IHC staining of Human breast cancer tissue paraffin-embedded, diluted at 1:200
      • The picture was kindly provided by our customer
      • The picture was kindly provided by our customer.
      • The picture was kindly provided by our customer.